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2wox

From Proteopedia

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Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa with NAD(P) H-catalytic thiol adduct.

Structural highlights

2wox is a 4 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BETB_PSEAE Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

PaBADH (Pseudomonas aeruginosa betaine aldehyde dehydrogenase) catalyses the irreversible NAD(P)+-dependent oxidation of betaine aldehyde to its corresponding acid, the osmoprotector glycine betaine. This reaction is involved in the catabolism of choline and in the response of this important pathogen to the osmotic and oxidative stresses prevalent in infection sites. The crystal structure of PaBADH in complex with NADPH showed a novel covalent adduct between the C2N of the pyridine ring and the sulfur atom of the catalytic cysteine residue, Cys286. This kind of adduct has not been reported previously either for a cysteine residue or for a low-molecular-mass thiol. The Michael addition of the cysteine thiolate in the 'resting' conformation to the double bond of the alpha,beta-unsaturated nicotinamide is facilitated by the particular conformation of NADPH in the active site of PaBADH (also observed in the crystal structure of the Cys286Ala mutant) and by an ordered water molecule hydrogen bonded to the carboxamide group. Reversible formation of NAD(P)H-Cys286 adducts in solution causes reversible enzyme inactivation as well as the loss of Cys286 reactivity towards thiol-specific reagents. This novel covalent modification may provide a physiologically relevant regulatory mechanism of the irreversible PaBADH-catalysed reaction, preventing deleterious decreases in the intracellular NAD(P)+/NAD(P)H ratios.

Novel NADPH-cysteine covalent adduct found in the active site of an aldehyde dehydrogenase.,Diaz-Sanchez AG, Gonzalez-Segura L, Rudino-Pinera E, Lira-Rocha A, Torres-Larios A, Munoz-Clares RA Biochem J. 2011 Nov 1;439(3):443-52. PMID:21732915^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Diaz-Sanchez AG, Gonzalez-Segura L, Rudino-Pinera E, Lira-Rocha A, Torres-Larios A, Munoz-Clares RA. Novel NADPH-cysteine covalent adduct found in the active site of an aldehyde dehydrogenase. Biochem J. 2011 Nov 1;439(3):443-52. PMID:21732915 doi:10.1042/BJ20110376
↑ Diaz-Sanchez AG, Gonzalez-Segura L, Rudino-Pinera E, Lira-Rocha A, Torres-Larios A, Munoz-Clares RA. Novel NADPH-cysteine covalent adduct found in the active site of an aldehyde dehydrogenase. Biochem J. 2011 Nov 1;439(3):443-52. PMID:21732915 doi:10.1042/BJ20110376

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wox"

@@ Line 1: / Line 1: @@
 ==Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa with NAD(P) H-catalytic thiol adduct.==
-<StructureSection load='2wox' size='340' side='right' caption='[[2wox]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='2wox' size='340' side='right'caption='[[2wox]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wox]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WOX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WOX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wox]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WOX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WOX FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7PE:2-(2-(2-(2-(2-(2-ETHOXYETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHANOL'>7PE</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wme|2wme]], [[2xek|2xek]], [[2xdr|2xdr]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7PE:2-(2-(2-(2-(2-(2-ETHOXYETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHANOL'>7PE</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Betaine-aldehyde_dehydrogenase Betaine-aldehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.8 1.2.1.8] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wox OCA], [https://pdbe.org/2wox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wox RCSB], [https://www.ebi.ac.uk/pdbsum/2wox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wox ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wox OCA], [http://pdbe.org/2wox PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wox RCSB], [http://www.ebi.ac.uk/pdbsum/2wox PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wox ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BETB_PSEAE BETB_PSEAE]] Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid.<ref>PMID:21732915</ref>
+[https://www.uniprot.org/uniprot/BETB_PSEAE BETB_PSEAE] Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid.<ref>PMID:21732915</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 31: @@
 ==See Also==
-*[[Aldehyde dehydrogenase|Aldehyde dehydrogenase]]
+*[[Aldehyde dehydrogenase 3D structures|Aldehyde dehydrogenase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Betaine-aldehyde dehydrogenase]]
+[[Category: Large Structures]]
-[[Category: Diaz-Sanchez, A G]]
+[[Category: Pseudomonas aeruginosa]]
-[[Category: Gonzalez-Segura, L]]
+[[Category: Diaz-Sanchez AG]]
-[[Category: Munoz-Clares, R A]]
+[[Category: Gonzalez-Segura L]]
-[[Category: Rudino-Pinera, E]]
+[[Category: Munoz-Clares RA]]
-[[Category: Aldehyde oxidation]]
+[[Category: Rudino-Pinera E]]
-[[Category: Nadph complex]]
-[[Category: Oxidoreductase]]

2wox

From Proteopedia

Current revision

Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa with NAD(P) H-catalytic thiol adduct.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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