2wp9

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[[Image:2wp9.png|left|200px]]
 
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==Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhB His207Thr mutant==
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The line below this paragraph, containing "STRUCTURE_2wp9", creates the "Structure Box" on the page.
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<StructureSection load='2wp9' size='340' side='right'caption='[[2wp9]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wp9]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WP9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBE:2-METHYL-N-PHENYL-5,6-DIHYDRO-1,4-OXATHIINE-3-CARBOXAMIDE'>CBE</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TEO:MALATE+LIKE+INTERMEDIATE'>TEO</scene></td></tr>
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{{STRUCTURE_2wp9| PDB=2wp9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp9 OCA], [https://pdbe.org/2wp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wp9 RCSB], [https://www.ebi.ac.uk/pdbsum/2wp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wp9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SDHA_ECOLI SDHA_ECOLI] Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.<ref>PMID:24374335</ref> <ref>PMID:12560550</ref> <ref>PMID:16407191</ref> <ref>PMID:19710024</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/2wp9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wp9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Succinate-ubiquinone oxidoreductase (SQR) and menaquinol-fumarate oxidoreductase (QFR) from Escherichia coli are members of the complex II family of enzymes. SQR and QFR catalyze similar reactions with quinones, however, SQR preferentially reacts with higher potential ubiquinones and QFR with lower potential napthoquinones. Both enzymes have a single functional quinone-binding site proximal to a [3Fe-4S] iron-sulfur cluster. A difference between SQR and QFR is that the redox potential of the [3Fe-4S] cluster in SQR is 140 mV higher than that found in QFR. This may reflect the character of the different quinones with which the two enzymes preferentially react. To investigate how the environment around the [3Fe-4S] cluster affects its redox properties and catalysis with quinones a conserved amino acid proximal to the cluster was mutated in both enzymes. It was found that substitution of SdhB His207 by threonine (like found in QFR) resulted in a 70 mV lowering of the redox potential of the cluster as measured by EPR. The converse mutation in QFR raised the redox potential of the cluster. X-ray structural analysis suggests that placing a charged residue near the [3Fe-4S] cluster is a primary reason for the alteration in redox potential with the hydrogen-bonding environment having a lesser effect. Steady state enzyme kinetic characterization of the mutant enzymes shows that the redox properties of the [3Fe-4S] cluster has only minor effect on catalysis.
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===CRYSTAL STRUCTURE OF THE E. COLI SUCCINATE:QUINONE OXIDOREDUCTASE (SQR) SDHB HIS207THR MUTANT===
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Perturbation of the quinone binding site of complex II alters the electronic properties of the proximal [3Fe-4S] iron-sulfur cluster.,Ruprecht J, Iwata S, Rothery RA, Weiner JH, Maklashina E, Cecchini G J Biol Chem. 2011 Feb 10. PMID:21310949<ref>PMID:21310949</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wp9" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21310949}}, adds the Publication Abstract to the page
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*[[Succinate dehydrogenase 3D structures|Succinate dehydrogenase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21310949 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21310949}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2wp9]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP9 OCA].
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==Reference==
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<ref group="xtra">PMID:21310949</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Cecchini, G.]]
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[[Category: Large Structures]]
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[[Category: Iwata, S.]]
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[[Category: Cecchini G]]
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[[Category: Maklashina, E.]]
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[[Category: Iwata S]]
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[[Category: Ruprecht, J.]]
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[[Category: Maklashina E]]
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[[Category: Yankovskaya, V.]]
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[[Category: Ruprecht J]]
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[[Category: Yankovskaya V]]

Current revision

Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhB His207Thr mutant

PDB ID 2wp9

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