2wqf

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{{Seed}}
 
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[[Image:2wqf.png|left|200px]]
 
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==Crystal Structure of the Nitroreductase CinD from Lactococcus lactis in Complex with FMN==
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The line below this paragraph, containing "STRUCTURE_2wqf", creates the "Structure Box" on the page.
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<StructureSection load='2wqf' size='340' side='right'caption='[[2wqf]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wqf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WQF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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{{STRUCTURE_2wqf| PDB=2wqf | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wqf OCA], [https://pdbe.org/2wqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wqf RCSB], [https://www.ebi.ac.uk/pdbsum/2wqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wqf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9CED0_LACLA Q9CED0_LACLA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wq/2wqf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wqf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In Lactococcus lactis IL1403, 14 genes are under the control of the copper-inducible CopR repressor. This so-called CopR regulon encompasses the CopR regulator, two putative CPx-type copper ATPases, a copper chaperone, and 10 additional genes of unknown function. We addressed here the function of one of these genes, ytjD, which we renamed cinD (copper-induced nitroreductase). Copper, cadmium, and silver induced cinD in vivo, as shown by real-time quantitative PCR. A knockout mutant of cinD was more sensitive to oxidative stress exerted by 4-nitroquinoline-N-oxide and copper. Purified CinD is a flavoprotein and reduced 2,6-dichlorophenolindophenol and 4-nitroquinoline-N-oxide with k(cat) values of 27 and 11 s(-1), respectively, using NADH as a reductant. CinD also exhibited significant catalase activity in vitro. The X-ray structure of CinD was resolved at 1.35 A and resembles those of other nitroreductases. CinD is thus a nitroreductase which can protect L. lactis against oxidative stress that could be exerted by nitroaromatic compounds and copper.
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===CRYSTAL STRUCTURE OF THE NITROREDUCTASE CIND FROM LACTOCOCCUS LACTIS IN COMPLEX WITH FMN===
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Structure and function of CinD (YtjD) of Lactococcus lactis, a copper-induced nitroreductase involved in defense against oxidative stress.,Mermod M, Mourlane F, Waltersperger S, Oberholzer AE, Baumann U, Solioz M J Bacteriol. 2010 Aug;192(16):4172-80. Epub 2010 Jun 18. PMID:20562311<ref>PMID:20562311</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wqf" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20562311}}, adds the Publication Abstract to the page
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*[[Nitroreductase 3D structures|Nitroreductase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20562311 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20562311}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2WQF is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQF OCA].
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==Reference==
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<ref group="xtra">PMID:20562311</ref><references group="xtra"/>
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[[Category: Lactococcus lactis]]
[[Category: Lactococcus lactis]]
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[[Category: Baumann, U.]]
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[[Category: Large Structures]]
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[[Category: Oberholzer, A E.]]
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[[Category: Baumann U]]
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[[Category: Solioz, M.]]
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[[Category: Oberholzer AE]]
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[[Category: Waltersperger, S M.]]
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[[Category: Solioz M]]
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[[Category: Copr regulated protein]]
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[[Category: Waltersperger SM]]
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[[Category: Nitroreductase]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Aug 12 00:03:46 2010''
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Current revision

Crystal Structure of the Nitroreductase CinD from Lactococcus lactis in Complex with FMN

PDB ID 2wqf

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