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2wry

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Crystal structure of chicken cytokine interleukin 1 beta

Structural highlights

2wry is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.58Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O73909_CHICK Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells (By similarity).[RuleBase:RU003753]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Interleukin-1 beta (IL-1beta) is an important cytokine in the immune system. The properties of avian IL-1betas are less well understood than the mammalian IL-1betas, and there is no available structure of avian IL-1betas in the Protein Data Bank. Here, we report the crystal structures of wild-type and Y157F mutant IL-1betas from chicken. Both the wild-type and mutant IL-1betas share a beta-trefoil conformation similar to that of human IL-1beta and also have an internal hydrophobic cavity. However, the cavity sizes clearly differ from that of human IL-1beta due to the packing of hydrophobic residues. Our studies also reveal that the relative thermal stability of IL-1betas does not correlate with cavity size but rather is dependent on the amino acid residues present around the cavity. This cavity serves as a scaffold for maintaining the structure of the IL-1beta core region but does not have a biological function per se. Moreover, we found that human IL-1beta cannot induce chemokine expression in chicken fibroblasts or elevate plasma cortisol levels in chickens, implying a lack of cross-species bioactivity. Close examination reveals that significant structural and sequence differences occur in the terminal and some loop regions between human and chicken IL-1betas. These variable regions have been shown to be critical for receptor binding, thus resulting in a lack of species cross-reactivity between human and chicken IL-1beta.

Structural and functional comparison of cytokine interleukin-1 beta from chicken and human.,Cheng CS, Chen WT, Lee LH, Chen YW, Chang SY, Lyu PC, Yin HS Mol Immunol. 2011 Mar;48(6-7):947-55. Epub 2011 Feb 1. PMID:21288573^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cheng CS, Chen WT, Lee LH, Chen YW, Chang SY, Lyu PC, Yin HS. Structural and functional comparison of cytokine interleukin-1 beta from chicken and human. Mol Immunol. 2011 Mar;48(6-7):947-55. Epub 2011 Feb 1. PMID:21288573 doi:10.1016/j.molimm.2011.01.002

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wry"

@@ Line 3: / Line 3: @@
 <StructureSection load='2wry' size='340' side='right'caption='[[2wry]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wry]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WRY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WRY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wry]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WRY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WRY FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wry OCA], [https://pdbe.org/2wry PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wry RCSB], [https://www.ebi.ac.uk/pdbsum/2wry PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wry ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wry OCA], [https://pdbe.org/2wry PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wry RCSB], [https://www.ebi.ac.uk/pdbsum/2wry PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wry ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/O73909_CHICK O73909_CHICK]] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells (By similarity).[RuleBase:RU003753]
+[https://www.uniprot.org/uniprot/O73909_CHICK O73909_CHICK] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells (By similarity).[RuleBase:RU003753]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 34: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Chick]]
+[[Category: Gallus gallus]]
 [[Category: Large Structures]]
-[[Category: Cheng, C S]]
+[[Category: Cheng CS]]
-[[Category: Lee, L H]]
+[[Category: Lee LH]]
-[[Category: Lu, W S]]
+[[Category: Lu WS]]
-[[Category: Lyu, P C]]
+[[Category: Lyu PC]]
-[[Category: Wang, W C]]
+[[Category: Wang WC]]
-[[Category: Yin, H S]]
+[[Category: Yin HS]]
-[[Category: Immune system]]
-[[Category: Inflammatory response]]
-[[Category: Mitogen]]
-[[Category: Pyrogen]]

2wry

From Proteopedia

Current revision

Crystal structure of chicken cytokine interleukin 1 beta

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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