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| - | ==CRYSTAL STRUCTURE OF THE RRM DOMAIN OF MOUSE DELETED IN AZOOSPERMIA-LIKE IN COMPLEX WITH MVH RNA, UGUUC== | + | ==Crystal structure of the RRM domain of mouse Deleted in azoospermia- like in complex with Mvh RNA, UGUUC== |
| - | <StructureSection load='2xs5' size='340' side='right' caption='[[2xs5]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='2xs5' size='340' side='right'caption='[[2xs5]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xs5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XS5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xs5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XS5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xs2|2xs2]], [[2xsf|2xsf]], [[2xs7|2xs7]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xs5 OCA], [http://pdbe.org/2xs5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xs5 RCSB], [http://www.ebi.ac.uk/pdbsum/2xs5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xs5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xs5 OCA], [https://pdbe.org/2xs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xs5 RCSB], [https://www.ebi.ac.uk/pdbsum/2xs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xs5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DAZL_MOUSE DAZL_MOUSE]] RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.<ref>PMID:22021443</ref> <ref>PMID:9288969</ref> | + | [https://www.uniprot.org/uniprot/DAZL_MOUSE DAZL_MOUSE] RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.<ref>PMID:22021443</ref> <ref>PMID:9288969</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Edwards, T A]] | + | [[Category: Mus musculus]] |
| - | [[Category: Jenkins, H T]] | + | [[Category: Edwards TA]] |
| - | [[Category: Rna binding protein-rna complex]] | + | [[Category: Jenkins HT]] |
| - | [[Category: Translation regulation]]
| + | |
| Structural highlights
Function
DAZL_MOUSE RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.[1] [2]
Publication Abstract from PubMed
A defect in germ-cell (sperm and oocyte) development is the leading cause of male and female infertility. Control of translation through the binding of deleted in azoospermia (DAZ)-like (DAZL) to the 3'-UTRs of mRNAs, via a highly conserved RNA recognition motif (RRM), has been shown to be essential in germ-cell development. Crystal structures of the RRM from murine DAZL (Dazl), both alone and in complex with RNA sequences from the 3'-UTRs of mRNAs regulated by Dazl, reveal high-affinity sequence-specific recognition of a GUU triplet involving an extended, kinked, pair of beta-strands. Recognition of the GUU triplet is maintained, whereas the identity and position of bases flanking this triplet varies. The Dazl RRM is thus able to recognize GUU triplets in different sequence contexts. Mutation of bases within the GUU triplet reduces the affinity of binding. Together with the demonstration that multiple Dazl RRMs can bind to a single RNA containing multiple GUU triplets, these structures suggest that the number of DAZL molecules bound to GUU triplets in the 3'-UTR provides a method for modulating the translation of a target RNA. The conservation of RNA binding and structurally important residues between members of the DAZ family, together with the demonstration that mutation of these residues severely impairs RNA binding, indicate that the mode of RNA binding revealed by these structures is conserved in proteins essential for gamete development from flies to humans.
Kinked {beta}-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL.,Jenkins HT, Malkova B, Edwards TA Proc Natl Acad Sci U S A. 2011 Oct 20. PMID:22021443[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jenkins HT, Malkova B, Edwards TA. Kinked {beta}-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL. Proc Natl Acad Sci U S A. 2011 Oct 20. PMID:22021443 doi:10.1073/pnas.1105211108
- ↑ Ruggiu M, Speed R, Taggart M, McKay SJ, Kilanowski F, Saunders P, Dorin J, Cooke HJ. The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis. Nature. 1997 Sep 4;389(6646):73-7. PMID:9288969 doi:http://dx.doi.org/10.1038/37987
- ↑ Jenkins HT, Malkova B, Edwards TA. Kinked {beta}-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL. Proc Natl Acad Sci U S A. 2011 Oct 20. PMID:22021443 doi:10.1073/pnas.1105211108
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