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| - | ==STRUCTURE OF N-TERMINAL DOMAIN OF CANDIDA ALBICANS ALS9-2 G299W MUTANT== | + | |
| - | <StructureSection load='2ylh' size='340' side='right' caption='[[2ylh]], [[Resolution|resolution]] 1.70Å' scene=''> | + | ==Structure of N-terminal domain of Candida albicans Als9-2 G299W mutant== |
| | + | <StructureSection load='2ylh' size='340' side='right'caption='[[2ylh]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ylh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YLH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ylh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YLH FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y7o|2y7o]], [[2y7l|2y7l]], [[2y7n|2y7n]], [[2y7m|2y7m]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ylh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ylh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ylh RCSB], [http://www.ebi.ac.uk/pdbsum/2ylh PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ylh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ylh OCA], [https://pdbe.org/2ylh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ylh RCSB], [https://www.ebi.ac.uk/pdbsum/2ylh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ylh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ALS9_CANAL ALS9_CANAL] Cell surface adhesion protein which mediates both yeast-to-host tissue adherence and yeast aggregation. Plays an important role in the pathogenesis of C.albicans infections (PubMed:17510860, PubMed:22321066, PubMed:22429754). Allele ALS9-2 contributes to endothelial cell adhesion, whereas ALS9-1 does not (PubMed:17600078).<ref>PMID:17510860</ref> <ref>PMID:17600078</ref> <ref>PMID:22321066</ref> <ref>PMID:22429754</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 2ylh" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Candida albicans]] | | [[Category: Candida albicans]] |
| - | [[Category: Burchell, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Cota, E]] | + | [[Category: Burchell L]] |
| - | [[Category: Salgado, P S]] | + | [[Category: Cota E]] |
| - | [[Category: Adhesin]] | + | [[Category: Salgado PS]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Peptide-binding protein]]
| + | |
| Structural highlights
Function
ALS9_CANAL Cell surface adhesion protein which mediates both yeast-to-host tissue adherence and yeast aggregation. Plays an important role in the pathogenesis of C.albicans infections (PubMed:17510860, PubMed:22321066, PubMed:22429754). Allele ALS9-2 contributes to endothelial cell adhesion, whereas ALS9-1 does not (PubMed:17600078).[1] [2] [3] [4]
Publication Abstract from PubMed
Candida albicans is the most prevalent fungal pathogen in humans and a major source of life-threatening nosocomial infections. The Als (agglutinin-like sequence) glycoproteins are an important virulence factor for this fungus and have been associated with binding of host-cell surface proteins and small peptides of random sequence, the formation of biofilms and amyloid fibers. High-resolution structures of N-terminal Als adhesins (NT-Als; up to 314 amino acids) show that ligand recognition relies on a motif capable of binding flexible C termini of peptides in extended conformation. Central to this mechanism is an invariant lysine that recognizes the C-terminal carboxylate of ligands at the end of a deep-binding cavity. In addition to several protein-peptide interactions, a network of water molecules runs parallel to one side of the ligand and contributes to the recognition of diverse peptide sequences. These data establish NT-Als adhesins as a separate family of peptide-binding proteins and an unexpected adhesion system for primary, widespread protein-protein interactions at the Candida/host-cell interface.
Structural basis for the broad specificity to host-cell ligands by the pathogenic fungus Candida albicans.,Salgado PS, Yan R, Taylor JD, Burchell L, Jones R, Hoyer LL, Matthews SJ, Simpson PJ, Cota E Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15775-9. Epub 2011 Sep 6. PMID:21896717[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Klotz SA, Gaur NK, De Armond R, Sheppard D, Khardori N, Edwards JE Jr, Lipke PN, El-Azizi M. Candida albicans Als proteins mediate aggregation with bacteria and yeasts. Med Mycol. 2007 Jun;45(4):363-70. doi: 10.1080/13693780701299333. PMID:17510860 doi:http://dx.doi.org/10.1080/13693780701299333
- ↑ Zhao X, Oh SH, Hoyer LL. Unequal contribution of ALS9 alleles to adhesion between Candida albicans and human vascular endothelial cells. Microbiology (Reading). 2007 Jul;153(Pt 7):2342-2350. doi:, 10.1099/mic.0.2006/005017-0. PMID:17600078 doi:http://dx.doi.org/10.1099/mic.0.2006/005017-0
- ↑ Aoki W, Kitahara N, Miura N, Morisaka H, Kuroda K, Ueda M. Profiling of adhesive properties of the agglutinin-like sequence (ALS) protein family, a virulent attribute of Candida albicans. FEMS Immunol Med Microbiol. 2012 Jun;65(1):121-4. doi:, 10.1111/j.1574-695X.2012.00941.x. Epub 2012 Mar 6. PMID:22321066 doi:http://dx.doi.org/10.1111/j.1574-695X.2012.00941.x
- ↑ Monroy-Perez E, Sainz-Espunes T, Paniagua-Contreras G, Negrete-Abascal E, Rodriguez-Moctezuma JR, Vaca S. Frequency and expression of ALS and HWP1 genotypes in Candida albicans strains isolated from Mexican patients suffering from vaginal candidosis. Mycoses. 2012 May;55(3):e151-7. doi: 10.1111/j.1439-0507.2012.02188.x. Epub 2012 , Mar 19. PMID:22429754 doi:http://dx.doi.org/10.1111/j.1439-0507.2012.02188.x
- ↑ Salgado PS, Yan R, Taylor JD, Burchell L, Jones R, Hoyer LL, Matthews SJ, Simpson PJ, Cota E. Structural basis for the broad specificity to host-cell ligands by the pathogenic fungus Candida albicans. Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15775-9. Epub 2011 Sep 6. PMID:21896717 doi:10.1073/pnas.1103496108
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