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2yn2
From Proteopedia
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| - | {{STRUCTURE_2yn2| PDB=2yn2 | SCENE= }} | ||
| - | ===Huf7p=== | ||
| - | == | + | ==Huf protein - paralogue of the tau55 histidine phosphatase domain== |
| - | [[2yn2]] is a 1 chain structure with sequence from [ | + | <StructureSection load='2yn2' size='340' side='right'caption='[[2yn2]], [[Resolution|resolution]] 2.05Å' scene=''> |
| - | [[ | + | == Structural highlights == |
| - | [[ | + | <table><tr><td colspan='2'>[[2yn2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YN2 FirstGlance]. <br> |
| - | [[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| - | [[Category: | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr> |
| - | [[Category: | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yn2 OCA], [https://pdbe.org/2yn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yn2 RCSB], [https://www.ebi.ac.uk/pdbsum/2yn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yn2 ProSAT]</span></td></tr> |
| - | [[Category: | + | </table> |
| - | [[Category: | + | == Function == |
| - | [[Category: | + | [https://www.uniprot.org/uniprot/YNK8_YEAST YNK8_YEAST] |
| - | [[Category: | + | <div style="background-color:#fffaf0;"> |
| - | [[Category: | + | == Publication Abstract from PubMed == |
| - | [[Category: | + | Saccharomyces cerevisiae tau55, a subunit of the RNA polymerase III-specific general transcription factor TFIIIC, comprises an N-terminal histidine phosphatase domain (tau55-HPD) whose catalytic activity and cellular function is poorly understood. We solved the crystal structures of tau55-HPD and its closely related paralogue Huf and used in silico docking methods to identify phospho-serine and phospho-tyrosine containing peptides as possible substrates that were subsequently validated using in vitro phosphatase assays. A comparative phospho-proteomic study identified additional phosphopeptides as possible targets, which show the involvement of these two phosphatases in the regulation of a variety of cellular functions. Our results identify tau55-HPD and Huf as bona fide protein phosphatases, characterize their substrate specificities and provide a small set of regulated phosphosite targets in vivo. |
| - | [[Category: | + | |
| - | [[Category: | + | Structural and functional characterization of a phosphatase domain within yeast general transcription factor TFIIIC.,Taylor NM, Glatt S, Hennrich ML, von Scheven G, Grotsch H, Fernandez-Tornero C, Rybin V, Gavin AC, Kolb P, Muller CW J Biol Chem. 2013 Apr 8. PMID:23569204<ref>PMID:23569204</ref> |
| - | [[Category: | + | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2yn2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Fernandez-Tornero C]] | ||
| + | [[Category: Gavin AC]] | ||
| + | [[Category: Glatt S]] | ||
| + | [[Category: Grotsch H]] | ||
| + | [[Category: Hennrich M]] | ||
| + | [[Category: Kolb P]] | ||
| + | [[Category: Muller CW]] | ||
| + | [[Category: Rybin V]] | ||
| + | [[Category: Taylor NMI]] | ||
| + | [[Category: Von Scheven G]] | ||
Current revision
Huf protein - paralogue of the tau55 histidine phosphatase domain
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