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4al9

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{{STRUCTURE_4al9| PDB=4al9 | SCENE= }}
 
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===CRystal structure of the lectin PA-IL from Pseudomonas aeruginoas in complex with melibiose===
 
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{{ABSTRACT_PUBMED_24123124}}
 
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==Function==
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==Crystal structure of the lectin PA-IL from Pseudomonas aeruginoas in complex with melibiose==
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[[http://www.uniprot.org/uniprot/PA1L_PSEAE PA1L_PSEAE]] D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.
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<StructureSection load='4al9' size='340' side='right'caption='[[4al9]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4al9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AL9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900037:alpha-melibiose'>PRD_900037</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4al9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4al9 OCA], [https://pdbe.org/4al9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4al9 RCSB], [https://www.ebi.ac.uk/pdbsum/4al9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4al9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA1L_PSEAE PA1L_PSEAE] D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The galactose-specific lectin LecA from Pseudomonas aeruginosa is a target for the development of new anti-infectious compounds. Sugar based molecules with anti-adhesive properties present great potential in the fight against bacterial infection and biofilm formation. LecA is specific for oligosaccharides with terminal alpha-galactoside residues and displays strong affinity for melibiose (alphaGal1-6Glc) with a Kd of 38.8 microM. The crystal structure of LecA/melibiose complex shows classical calcium-bridged binding of alphaGal in the primary binding site but also revealed a secondary sugar binding site with glucose bound. This sugar binding site is in close proximity to the galactose binding one, is independent of calcium and mainly involves interactions with a symmetry-related protein. This discovery would help to the design of new potent inhibitors targeting both binding sites.
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==About this Structure==
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Secondary sugar binding site identified for LecA lectin from Pseudomonas aeruginosa.,Blanchard B, Imberty A, Varrot A Proteins. 2013 Oct 1. doi: 10.1002/prot.24430. PMID:24123124<ref>PMID:24123124</ref>
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[[4al9]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AL9 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024123124</ref><references group="xtra"/><references/>
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</div>
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[[Category: Pseudomonas aeruginosa pao1]]
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<div class="pdbe-citations 4al9" style="background-color:#fffaf0;"></div>
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[[Category: Blanchard, B.]]
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[[Category: Imberty, A.]]
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==See Also==
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[[Category: Varrot, A.]]
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*[[Galactose-binding lectin|Galactose-binding lectin]]
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[[Category: Galactose binding protein]]
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== References ==
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[[Category: Sugar binding protein]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Blanchard B]]
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[[Category: Imberty A]]
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[[Category: Varrot A]]

Current revision

Crystal structure of the lectin PA-IL from Pseudomonas aeruginoas in complex with melibiose

PDB ID 4al9

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