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| | <StructureSection load='4ato' size='340' side='right'caption='[[4ato]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='4ato' size='340' side='right'caption='[[4ato]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ato]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ATO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ato]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ATO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ato FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ato OCA], [http://pdbe.org/4ato PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ato RCSB], [http://www.ebi.ac.uk/pdbsum/4ato PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ato ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ato FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ato OCA], [https://pdbe.org/4ato PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ato RCSB], [https://www.ebi.ac.uk/pdbsum/4ato PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ato ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TOXN_BACTK TOXN_BACTK] Toxic component of a type III toxin-antitoxin (TA) system (PubMed:19124776). An endoribonuclease which cleaves between the first and second A of AAAAA sequences; it tolerates other nucleotides in positions +2 and +4 of the consensus. Digests cognate antitoxin RNA ToxI as shown by the 2'-3'-cyclic phosphate at the 3' end of the 34-nt repeats and probably other RNAs (PubMed:23267117). Inhibits growth when expressed in E.coli without causing cell lysis; this bacteriostatic effect is neutralized by cognate RNA antitoxin ToxI, which has 2.9 nearly identical 34 nucleotide-long repeats (PubMed:19124776). Non-cognate antitoxin RNA from P.atrosepticum does not inhibit this toxin (PubMed:23267117). The toxin-antitoxin pair function in plasmid maintenance (a plasmid addiction system), but unlike its P.atrosepticum homolog it is not seen to confer resistance to bacteriophages (PubMed:23267117).<ref>PMID:19124776</ref> <ref>PMID:23267117</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus cereus var. thuringiensis smith et al. 1952]] | + | [[Category: Bacillus thuringiensis]] |
| - | [[Category: Blower, T R]] | + | [[Category: Large Structures]] |
| - | [[Category: Luisi, B F]] | + | [[Category: Blower TR]] |
| - | [[Category: Ong, S L]] | + | [[Category: Luisi BF]] |
| - | [[Category: Pei, X Y]] | + | [[Category: Ong SL]] |
| - | [[Category: Salmond, G P.C]] | + | [[Category: Pei XY]] |
| - | [[Category: Short, F L]] | + | [[Category: Salmond GPC]] |
| - | [[Category: Toxin-antitoxin complex]] | + | [[Category: Short FL]] |
| Structural highlights
Function
TOXN_BACTK Toxic component of a type III toxin-antitoxin (TA) system (PubMed:19124776). An endoribonuclease which cleaves between the first and second A of AAAAA sequences; it tolerates other nucleotides in positions +2 and +4 of the consensus. Digests cognate antitoxin RNA ToxI as shown by the 2'-3'-cyclic phosphate at the 3' end of the 34-nt repeats and probably other RNAs (PubMed:23267117). Inhibits growth when expressed in E.coli without causing cell lysis; this bacteriostatic effect is neutralized by cognate RNA antitoxin ToxI, which has 2.9 nearly identical 34 nucleotide-long repeats (PubMed:19124776). Non-cognate antitoxin RNA from P.atrosepticum does not inhibit this toxin (PubMed:23267117). The toxin-antitoxin pair function in plasmid maintenance (a plasmid addiction system), but unlike its P.atrosepticum homolog it is not seen to confer resistance to bacteriophages (PubMed:23267117).[1] [2]
Publication Abstract from PubMed
Bacterial small RNAs perform numerous regulatory roles, including acting as antitoxic components in toxin-antitoxin systems. In type III toxin-antitoxin systems, small processed RNAs directly antagonize their toxin protein partners, and in the systems characterized the toxin and antitoxin components together form a trimeric assembly. In the present study, we sought to define how the RNA antitoxin, ToxI, inhibits its potentially lethal protein partner, ToxN. We show through cross-inhibition experiments with the ToxIN systems from Pectobacterium atrosepticum (ToxIN(Pa)) and Bacillus thuringiensis (ToxIN(Bt)) that ToxI RNAs are highly selective enzyme inhibitors. Both systems have an "addictive" plasmid maintenance phenotype. We demonstrate that ToxI(Pa) can inhibit ToxN(Pa) in vitro both in its processed form and as a repetitive precursor RNA, and this inhibition is linked to the self-assembly of the trimeric complex. Inhibition and self-assembly are both mediated entirely by the ToxI(Pa) RNA, with no requirement for cellular factors or exogenous energy. Finally, we explain the origins of ToxI antitoxin selectivity through our crystal structure of the ToxIN(Bt) complex. Our results show how a processed RNA pseudoknot can inhibit a deleterious protein with exquisite molecular specificity and how these self-contained and addictive RNA-protein pairs can confer different adaptive benefits in their bacterial hosts.
Selectivity and self-assembly in the control of a bacterial toxin by an antitoxic noncoding RNA pseudoknot.,Short FL, Pei XY, Blower TR, Ong SL, Fineran PC, Luisi BF, Salmond GP Proc Natl Acad Sci U S A. 2012 Dec 24. PMID:23267117[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fineran PC, Blower TR, Foulds IJ, Humphreys DP, Lilley KS, Salmond GP. The phage abortive infection system, ToxIN, functions as a protein-RNA toxin-antitoxin pair. Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):894-9. Epub 2009 Jan 5. PMID:19124776 doi:10.1073/pnas.0808832106
- ↑ Short FL, Pei XY, Blower TR, Ong SL, Fineran PC, Luisi BF, Salmond GP. Selectivity and self-assembly in the control of a bacterial toxin by an antitoxic noncoding RNA pseudoknot. Proc Natl Acad Sci U S A. 2012 Dec 24. PMID:23267117 doi:http://dx.doi.org/10.1073/pnas.1216039110
- ↑ Short FL, Pei XY, Blower TR, Ong SL, Fineran PC, Luisi BF, Salmond GP. Selectivity and self-assembly in the control of a bacterial toxin by an antitoxic noncoding RNA pseudoknot. Proc Natl Acad Sci U S A. 2012 Dec 24. PMID:23267117 doi:http://dx.doi.org/10.1073/pnas.1216039110
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