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4av7

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==Structure determination of the double mutant S233Y F250G from the sec- alkyl sulfatase PisA1==
==Structure determination of the double mutant S233Y F250G from the sec- alkyl sulfatase PisA1==
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<StructureSection load='4av7' size='340' side='right' caption='[[4av7]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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<StructureSection load='4av7' size='340' side='right'caption='[[4av7]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4av7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._dsm_6611 Pseudomonas sp. dsm 6611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AV7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AV7 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4av7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._DSM_6611 Pseudomonas sp. DSM 6611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AV7 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yhe|2yhe]], [[4axh|4axh]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4av7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4av7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4av7 RCSB], [http://www.ebi.ac.uk/pdbsum/4av7 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4av7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4av7 OCA], [https://pdbe.org/4av7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4av7 RCSB], [https://www.ebi.ac.uk/pdbsum/4av7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4av7 ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RSAKS_PSESP RSAKS_PSESP] Alkylsulfatase that catalyzes the enantioselective hydrolysis of secondary-alkylsulfates with strict inversion of configuration, leading to the formation of homochiral (S)-configurated alcohols and nonreacted sulfate esters (PubMed:21770430, Ref.2, PubMed:23061549). The substrate spectrum includes a range of linear, branched or cyclic sec-alkylsulfates (PubMed:21770430). Can use sec-alkylsulfate esters bearing aromatic, olefinic and acetylenic moieties (Ref.2). Acts by cleaving the C-O bond, resulting in inversion at the carbon (PubMed:23061549).<ref>PMID:21770430</ref> <ref>PMID:23061549</ref> <ref>PMID:21770430</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
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<div class="pdbe-citations 4av7" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Sulfatase 3D structures|Sulfatase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pseudomonas sp. dsm 6611]]
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[[Category: Large Structures]]
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[[Category: Faber, K]]
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[[Category: Pseudomonas sp. DSM 6611]]
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[[Category: Knaus, T]]
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[[Category: Faber K]]
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[[Category: Macharaux, P]]
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[[Category: Knaus T]]
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[[Category: Schober, M]]
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[[Category: Macharaux P]]
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[[Category: Wagner, U]]
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[[Category: Schober M]]
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[[Category: Double mutant]]
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[[Category: Wagner U]]
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[[Category: Hydrolase]]
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[[Category: Inverting alkylsulfatase]]
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Current revision

Structure determination of the double mutant S233Y F250G from the sec- alkyl sulfatase PisA1

PDB ID 4av7

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