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4bj3

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{{STRUCTURE_4bj3| PDB=4bj3 | SCENE= }}
 
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===Integrin alpha2 I domain E318W-collagen complex===
 
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{{ABSTRACT_PUBMED_23922814}}
 
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==Function==
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==Integrin alpha2 I domain E318W-collagen complex==
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[[http://www.uniprot.org/uniprot/ITA2_HUMAN ITA2_HUMAN]] Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
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<StructureSection load='4bj3' size='340' side='right'caption='[[4bj3]], [[Resolution|resolution]] 3.04&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4bj3]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BJ3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.042&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bj3 OCA], [https://pdbe.org/4bj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bj3 RCSB], [https://www.ebi.ac.uk/pdbsum/4bj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bj3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ITA2_HUMAN ITA2_HUMAN] Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The GFOGER motif in collagens (O denotes hydroxyproline) represents a high-affinity binding site for all collagen-binding integrins. Other GxOGER motifs require integrin activation for maximal binding. The E318W mutant of the integrin alpha2beta1 I domain displays a relaxed collagen specificity, typical of an active state. E318W binds more strongly than the wild-type alpha2 I domain to GMOGER, and forms a 2:1 complex with a homotrimeric, collagen-like, GFOGER peptide. Crystal structure analysis of this complex reveals two E318W I domains, A and B, bound to a single triple helix. The E318W I domains are virtually identical to the collagen-bound wild-type I domain, suggesting that the E318W mutation activates the I domain by destabilising the unligated conformation. E318W I domain A interacts with two collagen chains similarly to wild-type I domain (high-affinity mode). E318W I domain B makes favourable interactions with only one collagen chain (low-affinity mode). This observation suggests that single GxOGER motifs in the heterotrimeric collagens V and IX may support binding of activated integrins.
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==About this Structure==
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An activating mutation reveals a second binding mode of the integrin alpha2 I domain to the GFOGER motif in collagens.,Carafoli F, Hamaia SW, Bihan D, Hohenester E, Farndale RW PLoS One. 2013 Jul 29;8(7):e69833. doi: 10.1371/journal.pone.0069833. Print 2013. PMID:23922814<ref>PMID:23922814</ref>
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[[4bj3]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJ3 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023922814</ref><references group="xtra"/><references/>
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</div>
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[[Category: Bihan, D.]]
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<div class="pdbe-citations 4bj3" style="background-color:#fffaf0;"></div>
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[[Category: Carafoli, F.]]
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[[Category: Farndale, R W.]]
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==See Also==
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[[Category: Hamaia, S W.]]
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*[[Integrin 3D structures|Integrin 3D structures]]
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[[Category: Hohenester, E.]]
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== References ==
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[[Category: Cell adhesion]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Bihan D]]
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[[Category: Carafoli F]]
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[[Category: Farndale RW]]
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[[Category: Hamaia SW]]
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[[Category: Hohenester E]]

Current revision

Integrin alpha2 I domain E318W-collagen complex

PDB ID 4bj3

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