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4bwp

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Structure of Drosophila Melanogaster PAN3 pseudokinase

Structural highlights

4bwp is a 2 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PAN3_DROME Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.[HAMAP-Rule:MF_03181]^[1]

Publication Abstract from PubMed

The PAN2-PAN3 deadenylase complex functions in general and miRNA-mediated mRNA degradation and is specifically recruited to miRNA targets by GW182/TNRC6 proteins. We describe the PAN3 adaptor protein crystal structure that, unexpectedly, forms intertwined and asymmetric homodimers. Dimerization is mediated by a coiled coil that links an N-terminal pseudokinase to a C-terminal knob domain. The PAN3 pseudokinase binds ATP, and this function is required for mRNA degradation in vivo. We further identified conserved surfaces required for mRNA degradation, including the binding surface for the PAN2 deadenylase on the knob domain. The most remarkable structural feature is the presence of a tryptophan-binding pocket at the dimer interface, which mediates binding to TNRC6C in human cells. Together, our data reveal the structural basis for the interaction of PAN3 with PAN2 and the recruitment of the PAN2-PAN3 complex to miRNA targets by TNRC6 proteins.

Structure of the PAN3 Pseudokinase Reveals the Basis for Interactions with the PAN2 Deadenylase and the GW182 Proteins.,Christie M, Boland A, Huntzinger E, Weichenrieder O, Izaurralde E Mol Cell. 2013 Aug 8;51(3):360-73. doi: 10.1016/j.molcel.2013.07.011. PMID:23932717^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Huntzinger E, Kuzuoglu-Ozturk D, Braun JE, Eulalio A, Wohlbold L, Izaurralde E. The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets. Nucleic Acids Res. 2013 Jan;41(2):978-94. doi: 10.1093/nar/gks1078. Epub 2012 Nov, 21. PMID:23172285 doi:http://dx.doi.org/10.1093/nar/gks1078
↑ Christie M, Boland A, Huntzinger E, Weichenrieder O, Izaurralde E. Structure of the PAN3 Pseudokinase Reveals the Basis for Interactions with the PAN2 Deadenylase and the GW182 Proteins. Mol Cell. 2013 Aug 8;51(3):360-73. doi: 10.1016/j.molcel.2013.07.011. PMID:23932717 doi:10.1016/j.molcel.2013.07.011

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bwp"

@@ Line 1: / Line 1: @@
 ==Structure of Drosophila Melanogaster PAN3 pseudokinase==
-<StructureSection load='4bwp' size='340' side='right' caption='[[4bwp]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
+<StructureSection load='4bwp' size='340' side='right'caption='[[4bwp]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4bwp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BWP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4bwp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BWP FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AN2:AMP+PHOSPHORAMIDATE'>AN2</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bwk|4bwk]], [[4bwx|4bwx]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AN2:AMP+PHOSPHORAMIDATE'>AN2</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwp OCA], [http://pdbe.org/4bwp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bwp RCSB], [http://www.ebi.ac.uk/pdbsum/4bwp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwp ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwp OCA], [https://pdbe.org/4bwp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bwp RCSB], [https://www.ebi.ac.uk/pdbsum/4bwp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwp ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PAN3_DROME PAN3_DROME] Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.[HAMAP-Rule:MF_03181]<ref>PMID:23172285</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 21: @@
 ==See Also==
-*[[Ribonuclease|Ribonuclease]]
+*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
-[[Category: Boland, A]]
+[[Category: Large Structures]]
-[[Category: Christie, M]]
+[[Category: Boland A]]
-[[Category: Huntzinger, E]]
+[[Category: Christie M]]
-[[Category: Izaurralde, E]]
+[[Category: Huntzinger E]]
-[[Category: Weichenrieder, O]]
+[[Category: Izaurralde E]]
-[[Category: Deadenylase]]
+[[Category: Weichenrieder O]]
-[[Category: Gene regulation]]
-[[Category: Mirna]]
-[[Category: Mrna decay]]
-[[Category: Pan2]]

4bwp

From Proteopedia

Current revision

Structure of Drosophila Melanogaster PAN3 pseudokinase

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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