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4u7b

From Proteopedia

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Crystal structure of a pre-cleavage Mos1 transpososome

Structural highlights

4u7b is a 9 chain structure with sequence from Drosophila mauritiana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.09Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MOS1T_DROMA Mediates transposition of transposon Mos1 by a 'cut and paste' mechanism. Transposases are sequence-specific nucleases and strand transferases that catalyze transposition through an ordered series of events: sequence-specific binding of transposase to the terminal inverted repeats (IR) present at each end of the transposon, pairing of the transposon IRs in a paired-end complex (PEC), cleavage of one or both DNA strands at each transposon end, capture of target DNA, and strand transfer to insert the transposon at a new site.

Publication Abstract from PubMed

During cut-and-paste mariner/Tc1 transposition, transposon DNA is cut precisely at its junction with flanking DNA, ensuring the transposon is neither shortened nor lengthened with each transposition event. Each transposon end is flanked by a TpA dinucleotide: the signature target site duplication of mariner/Tc1 transposition. To establish the role of this sequence in accurate DNA cleavage, we have determined the crystal structure of a pre-second strand cleavage mariner Mos1 transpososome. The structure reveals the route of an intact DNA strand through the transposase active site before second strand cleavage. The crossed architecture of this pre-second strand cleavage paired-end complex supports our proposal that second strand cleavage occurs in trans. The conserved mariner transposase WVPHEL and YSPDL motifs position the strand for accurate DNA cleavage. Base-specific recognition of the flanking DNA by conserved amino acids is revealed, defining a new role for the WVPHEL motif in mariner transposition and providing a molecular explanation for in vitro mutagenesis data. Comparison of the pre-TS cleavage and post-cleavage Mos1 transpososomes with structures of Prototype Foamy Virus intasomes suggests a binding mode for target DNA prior to Mos1 transposon integration.

Structural role of the flanking DNA in mariner transposon excision.,Dornan J, Grey H, Richardson JM Nucleic Acids Res. 2015 Feb 8. pii: gkv096. PMID:25662605^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dornan J, Grey H, Richardson JM. Structural role of the flanking DNA in mariner transposon excision. Nucleic Acids Res. 2015 Feb 8. pii: gkv096. PMID:25662605 doi:http://dx.doi.org/10.1093/nar/gkv096

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4u7b"

Categories: Drosophila mauritiana | Large Structures | Richardson JM

@@ Line 3: / Line 3: @@
 <StructureSection load='4u7b' size='340' side='right'caption='[[4u7b]], [[Resolution|resolution]] 3.09&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4u7b]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Droma Droma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U7B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U7B FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4u7b]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_mauritiana Drosophila mauritiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U7B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U7B FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.09&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hos|3hos]], [[3hot|3hot]], [[2f7t|2f7t]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">marinerT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7226 DROMA])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u7b OCA], [https://pdbe.org/4u7b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u7b RCSB], [https://www.ebi.ac.uk/pdbsum/4u7b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u7b ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u7b OCA], [http://pdbe.org/4u7b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u7b RCSB], [http://www.ebi.ac.uk/pdbsum/4u7b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u7b ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MOS1T_DROMA MOS1T_DROMA]] Mediates transposition of transposon Mos1 by a 'cut and paste' mechanism. Transposases are sequence-specific nucleases and strand transferases that catalyze transposition through an ordered series of events: sequence-specific binding of transposase to the terminal inverted repeats (IR) present at each end of the transposon, pairing of the transposon IRs in a paired-end complex (PEC), cleavage of one or both DNA strands at each transposon end, capture of target DNA, and strand transfer to insert the transposon at a new site.
+[https://www.uniprot.org/uniprot/MOS1T_DROMA MOS1T_DROMA] Mediates transposition of transposon Mos1 by a 'cut and paste' mechanism. Transposases are sequence-specific nucleases and strand transferases that catalyze transposition through an ordered series of events: sequence-specific binding of transposase to the terminal inverted repeats (IR) present at each end of the transposon, pairing of the transposon IRs in a paired-end complex (PEC), cleavage of one or both DNA strands at each transposon end, capture of target DNA, and strand transfer to insert the transposon at a new site.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 ==See Also==
-*[[Transposase|Transposase]]
+*[[Transposase 3D structures|Transposase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Droma]]
+[[Category: Drosophila mauritiana]]
 [[Category: Large Structures]]
-[[Category: Richardson, J M]]
+[[Category: Richardson JM]]
-[[Category: Dna transposition]]
-[[Category: Helix-turn-helix]]
-[[Category: Hydrolase-dna complex]]
-[[Category: Rnase-h like fold]]
-[[Category: Transposase]]
-[[Category: Transposon synaptic complex]]

4u7b

From Proteopedia

Current revision

Crystal structure of a pre-cleavage Mos1 transpososome

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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