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2ns3

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[[Image:2ns3.jpg|left|200px]]
 
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{{Structure
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==Solution structure of ribbon BuIA==
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|PDB= 2ns3 |SIZE=350|CAPTION= <scene name='initialview01'>2ns3</scene>
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<StructureSection load='2ns3' size='340' side='right'caption='[[2ns3]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>
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<table><tr><td colspan='2'>[[2ns3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_bullatus Conus bullatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NS3 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ns3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns3 OCA], [https://pdbe.org/2ns3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ns3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ns3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns3 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ns3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns3 OCA], [http://www.ebi.ac.uk/pdbsum/2ns3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ns3 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CA1A_CONBU CA1A_CONBU] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This peptide potently blocks numerous mammalian nAChR subtypes (alpha-6 or -3/beta-2 or -3 > alpha-6 or-3/beta-4 > alpha-3/beta-2 > alpha-3/beta-4 > alpha-4/beta-4 = alpha-2/beta-4 > alpha-7 > alpha-2/beta-2 >> alpha-4/beta-2). Recovery from toxin block is markedly slower for beta-4 versus beta-2 subunit-containing nAChRs. Thus, it represents a novel probe for distinguishing between beta-2 and beta-4-containing nAChRs.
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<div style="background-color:#fffaf0;">
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'''Solution structure of ribbon BuIA'''
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== Publication Abstract from PubMed ==
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==Overview==
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BACKGROUND: Alpha-conotoxins have exciting therapeutic potential based on their high selectivity and affinity for nicotinic acetylcholine receptors. The spacing between the cysteine residues in alpha-conotoxins is variable, leading to the classification of sub-families. BuIA is the only alpha-conotoxin containing a 4/4 cysteine spacing and thus it is of significant interest to examine the structure of this conotoxin. RESULTS: In the current study we show the native globular disulfide connectivity of BuIA displays multiple conformations in solution whereas the non-native ribbon isomer has a single well-defined conformation. Despite having multiple conformations in solution the globular form of BuIA displays activity at the nicotinic acetylcholine receptor, contrasting with the lack of activity of the structurally well-defined ribbon isomer. CONCLUSION: These findings are opposite to the general trends observed for alpha-conotoxins where the native isomers have well-defined structures and the ribbon isomers are generally disordered. This study thus highlights the influence of the disulfide connectivity of BuIA on the dynamics of the three-dimensional structure.
BACKGROUND: Alpha-conotoxins have exciting therapeutic potential based on their high selectivity and affinity for nicotinic acetylcholine receptors. The spacing between the cysteine residues in alpha-conotoxins is variable, leading to the classification of sub-families. BuIA is the only alpha-conotoxin containing a 4/4 cysteine spacing and thus it is of significant interest to examine the structure of this conotoxin. RESULTS: In the current study we show the native globular disulfide connectivity of BuIA displays multiple conformations in solution whereas the non-native ribbon isomer has a single well-defined conformation. Despite having multiple conformations in solution the globular form of BuIA displays activity at the nicotinic acetylcholine receptor, contrasting with the lack of activity of the structurally well-defined ribbon isomer. CONCLUSION: These findings are opposite to the general trends observed for alpha-conotoxins where the native isomers have well-defined structures and the ribbon isomers are generally disordered. This study thus highlights the influence of the disulfide connectivity of BuIA on the dynamics of the three-dimensional structure.
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==About this Structure==
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Structure of alpha-conotoxin BuIA: influences of disulfide connectivity on structural dynamics.,Jin AH, Brandstaetter H, Nevin ST, Tan CC, Clark RJ, Adams DJ, Alewood PF, Craik DJ, Daly NL BMC Struct Biol. 2007 Apr 20;7:28. PMID:17445276<ref>PMID:17445276</ref>
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2NS3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS3 OCA].
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==Reference==
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Structure of alpha-conotoxin BuIA: influences of disulfide connectivity on structural dynamics., Jin AH, Brandstaetter H, Nevin ST, Tan CC, Clark RJ, Adams DJ, Alewood PF, Craik DJ, Daly NL, BMC Struct Biol. 2007 Apr 20;7:28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17445276 17445276]
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[[Category: Single protein]]
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[[Category: Adams, D J.]]
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[[Category: Alewood, P F.]]
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[[Category: Brandstaetter, H.]]
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[[Category: Clark, R J.]]
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[[Category: Craik, D J.]]
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[[Category: Daly, N L.]]
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[[Category: Jin, A H.]]
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[[Category: Nevin, S T.]]
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[[Category: Tan, C C.]]
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[[Category: ribbon disulfide connectivity]]
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[[Category: toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:07:32 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ns3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Conus bullatus]]
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[[Category: Large Structures]]
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[[Category: Adams DJ]]
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[[Category: Alewood PF]]
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[[Category: Brandstaetter H]]
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[[Category: Clark RJ]]
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[[Category: Craik DJ]]
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[[Category: Daly NL]]
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[[Category: Jin AH]]
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[[Category: Nevin ST]]
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[[Category: Tan CC]]

Current revision

Solution structure of ribbon BuIA

PDB ID 2ns3

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