This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1b8z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1b8z" [edit=sysop:move=sysop])
Current revision (23:21, 27 December 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1b8z.png|left|200px]]
 
-
{{STRUCTURE_1b8z| PDB=1b8z | SCENE= }}
+
==HU FROM THERMOTOGA MARITIMA==
 +
<StructureSection load='1b8z' size='340' side='right'caption='[[1b8z]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1b8z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B8Z FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b8z OCA], [https://pdbe.org/1b8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b8z RCSB], [https://www.ebi.ac.uk/pdbsum/1b8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b8z ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DBH_THEMA DBH_THEMA] Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/1b8z_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b8z ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The humar gene encoding for the histone-like DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima was efficiently overexpressed in Escherichia coli under the T7 promoter. The HU protein was purified using SP-Sepharose ion-exchange and heparin-affinity chromatography and was successfully crystallized in ammonium sulfate. The crystals were grown in the tetragonal form in space group P43 or P41 and have unit-cell dimensions a = b = 46.12, c = 77.56 A, alpha = beta = gamma = 90 degrees. The crystals diffract X-rays to 1.6 A resolution using synchrotron radiation and are suitable for determination of the HU structure at high resolution.
-
===HU FROM THERMOTOGA MARITIMA===
+
Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima.,Christodoulou E, Vorgias CE Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):1043-5. PMID:9757133<ref>PMID:9757133</ref>
-
{{ABSTRACT_PUBMED_9757133}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 1b8z" style="background-color:#fffaf0;"></div>
-
[[1b8z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8Z OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:009757133</ref><ref group="xtra">PMID:015048824</ref><references group="xtra"/>
+
</StructureSection>
-
[[Category: Thermotoga maritima]]
+
[[Category: Large Structures]]
-
[[Category: Christodoulou, E.]]
+
-
[[Category: Rypniewski, W R.]]
+
-
[[Category: Vorgias, C E.]]
+
-
[[Category: Dna binding protein]]
+
-
[[Category: Thermostable dna binding protein]]
+
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
 +
[[Category: Christodoulou E]]
 +
[[Category: Rypniewski WR]]
 +
[[Category: Vorgias CE]]

Current revision

HU FROM THERMOTOGA MARITIMA

PDB ID 1b8z

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1b8z"
Personal tools