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1cjg

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NMR STRUCTURE OF LAC REPRESSOR HP62-DNA COMPLEX

Structural highlights

1cjg is a 4 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LACI_ECOLI Repressor of the lactose operon. Binds allolactose as an inducer.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

BACKGROUND: Lactose repressor protein (Lac) controls the expression of the lactose metabolic genes in Escherichia coli by binding to an operator sequence in the promoter of the lac operon. Binding of inducer molecules to the Lac core domain induces changes in tertiary structure that are propagated to the DNA-binding domain through the connecting hinge region, thereby reducing the affinity for the operator. Protein-protein and protein-DNA interactions involving the hinge region play a crucial role in the allosteric changes occurring upon induction, but have not, as yet, been analyzed in atomic detail. RESULTS: We have used nuclear magnetic resonance (NMR) spectroscopy and restrained molecular dynamics (rMD) to determine the structure of the Lac repressor DNA-binding domain (headpeice 62; HP62) in complex with a symmetrized lac operator. Analysis of the structures reveals specific interactions between Lac repressor and DNA that were not found in previously investigated Lac repressor-DNA complexes. Important differences with the previously reported structures of the HP56-DNA complex were found in the loop following the helix-turn-helix (HTH) motif. The protein-protein and protein-DNA interactions involving the hinge region and the deformations in the DNA structure could be delineated in atomic detail. The structures were also used for comparison with the available crystallographic data on the Lac and Pur repressor-DNA complexes. CONCLUSIONS: The structures of the HP62-DNA complex provide the basis for a better understanding of the specific recognition in the Lac repressor-operator complex. In addition, the structural features of the hinge region provide detailed insight into the protein-protein and protein-DNA interactions responsible for the high affinity of the repressor for operator DNA.

The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator.,Spronk CA, Bonvin AM, Radha PK, Melacini G, Boelens R, Kaptein R Structure. 1999 Dec 15;7(12):1483-92. PMID:10647179^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Spronk CA, Bonvin AM, Radha PK, Melacini G, Boelens R, Kaptein R. The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator. Structure. 1999 Dec 15;7(12):1483-92. PMID:10647179

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cjg"

@@ Line 1: / Line 1: @@
 ==NMR STRUCTURE OF LAC REPRESSOR HP62-DNA COMPLEX==
-<StructureSection load='1cjg' size='340' side='right'caption='[[1cjg]], [[NMR_Ensembles_of_Models | 11 NMR models]]' scene=''>
+<StructureSection load='1cjg' size='340' side='right'caption='[[1cjg]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1cjg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CJG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1cjg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CJG FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LAC I, THE PART ENCODING THE 62 N-TERMINAL AMINOACIDS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cjg OCA], [https://pdbe.org/1cjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cjg RCSB], [https://www.ebi.ac.uk/pdbsum/1cjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cjg ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI]] Repressor of the lactose operon. Binds allolactose as an inducer.
+[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Boelens, R]]
+[[Category: Boelens R]]
-[[Category: Bonvin, A M.J J]]
+[[Category: Bonvin AMJJ]]
-[[Category: Kaptein, R]]
+[[Category: Kaptein R]]
-[[Category: Melacini, G]]
+[[Category: Melacini G]]
-[[Category: Radha, P K]]
+[[Category: Radha PK]]
-[[Category: Spronk, C A.E M]]
+[[Category: Spronk CAEM]]
-[[Category: Headpiece]]
-[[Category: Lac operator]]
-[[Category: Lac operon]]
-[[Category: Lac repressor]]
-[[Category: Transcription regulation]]
-[[Category: Transcription-dna complex]]

1cjg

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Current revision

NMR STRUCTURE OF LAC REPRESSOR HP62-DNA COMPLEX

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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