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1iub

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(New page: 200px<br /><applet load="1iub" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iub, resolution 2.31&Aring;" /> '''Fucose-specific lect...)
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[[Image:1iub.jpg|left|200px]]<br /><applet load="1iub" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1iub, resolution 2.31&Aring;" />
 
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'''Fucose-specific lectin from Aleuria aurantia (Hg-derivative form)'''<br />
 
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==Overview==
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==Fucose-specific lectin from Aleuria aurantia (Hg-derivative form)==
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Aleuria aurantia possesses a fucose-specific lectin (AAL) that is widely, used as a specific probe for fucose. Fucosylated sugars often play pivotal, roles in many cellular processes. We have determined the crystal structure, of AAL at 2.24 A resolution in complex with only three fucose molecules in, its five sugar binding sites of a six-fold beta-propeller structure. Very, recently, the structure of AAL has been independently determined, showing, that all the five binding sites were occupied by fucose molecules, [Wimmerova, M., et al. (2003) J. Biol. Chem. 278, 27059-27067]., Stabilization of the arginine conformation bound to fucose molecules plays, an essential role in generating the difference in the affinity in the five, binding sites. Binding models with a couple of saccharides based on, biochemical assays suggest that hydrophobic contacts also play important, roles in AAL recognizing its ligand.
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<StructureSection load='1iub' size='340' side='right'caption='[[1iub]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1iub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IUB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iub OCA], [https://pdbe.org/1iub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iub RCSB], [https://www.ebi.ac.uk/pdbsum/1iub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iub ProSAT], [https://www.topsan.org/Proteins/RSGI/1iub TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LECF_ALEAU LECF_ALEAU] Might have a role in the differentiation of cells. Binds L-fucose.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/1iub_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iub ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Aleuria aurantia possesses a fucose-specific lectin (AAL) that is widely used as a specific probe for fucose. Fucosylated sugars often play pivotal roles in many cellular processes. We have determined the crystal structure of AAL at 2.24 A resolution in complex with only three fucose molecules in its five sugar binding sites of a six-fold beta-propeller structure. Very recently, the structure of AAL has been independently determined, showing that all the five binding sites were occupied by fucose molecules [Wimmerova, M., et al. (2003) J. Biol. Chem. 278, 27059-27067]. Stabilization of the arginine conformation bound to fucose molecules plays an essential role in generating the difference in the affinity in the five binding sites. Binding models with a couple of saccharides based on biochemical assays suggest that hydrophobic contacts also play important roles in AAL recognizing its ligand.
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==About this Structure==
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Crystal structure of fucose-specific lectin from Aleuria aurantia binding ligands at three of its five sugar recognition sites.,Fujihashi M, Peapus DH, Kamiya N, Nagata Y, Miki K Biochemistry. 2003 Sep 30;42(38):11093-9. PMID:14503859<ref>PMID:14503859</ref>
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1IUB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia] with FUL, SO4, CL and HG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IUB OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of fucose-specific lectin from Aleuria aurantia binding ligands at three of its five sugar recognition sites., Fujihashi M, Peapus DH, Kamiya N, Nagata Y, Miki K, Biochemistry. 2003 Sep 30;42(38):11093-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14503859 14503859]
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</div>
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<div class="pdbe-citations 1iub" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Aleuria aurantia]]
[[Category: Aleuria aurantia]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fujihashi, M.]]
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[[Category: Fujihashi M]]
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[[Category: Kamiya, N.]]
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[[Category: Kamiya N]]
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[[Category: Miki, K.]]
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[[Category: Miki K]]
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[[Category: Nagata, Y.]]
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[[Category: Nagata Y]]
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[[Category: Peapus, D.H.]]
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[[Category: Peapus DH]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: CL]]
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[[Category: FUL]]
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[[Category: HG]]
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[[Category: SO4]]
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[[Category: hg]]
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[[Category: lectin]]
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[[Category: mad]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:40:28 2007''
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Current revision

Fucose-specific lectin from Aleuria aurantia (Hg-derivative form)

PDB ID 1iub

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