1v2z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (23:56, 27 December 2023) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1v2z.jpg|left|200px]]
 
-
<!--
+
==Crystal structure of the C-terminal domain of Thermosynechococcus elongatus BP-1 KaiA==
-
The line below this paragraph, containing "STRUCTURE_1v2z", creates the "Structure Box" on the page.
+
<StructureSection load='1v2z' size='340' side='right'caption='[[1v2z]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1v2z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V2Z FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2z OCA], [https://pdbe.org/1v2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v2z RCSB], [https://www.ebi.ac.uk/pdbsum/1v2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v2z ProSAT], [https://www.topsan.org/Proteins/RSGI/1v2z TOPSAN]</span></td></tr>
-
{{STRUCTURE_1v2z| PDB=1v2z | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KAIA_THEVB KAIA_THEVB] Key component of the KaiABC oscillator complex, which constitutes the main circadian regulator in cyanobacteria. Complex composition changes during the circadian cycle to control KaiC phosphorylation. KaiA stimulates KaiC autophosphorylation, while KaiB sequesters KaiA, leading to KaiC autodephosphorylation. KaiA binding to the KaiC CII domain during the subjective day yields KaiA(2-4):KaiC(6) complexes which stimulate KaiC autophosphorylation. Phospho-Ser-431 KaiC accumulation triggers binding of KaiB during the subjective night to form the KaiB(6):KaiC(6) complex, leading to changes in the output regulators CikA and SasA. KaiB(6):KaiC(6) formation exposes a site for KaiA binding on KaiB that sequesters KaiA from KaiC's CII domain, making the KaiC(6):KaiB(6):KaiA(12) complex resulting in KaiC autodephosphorylation. Complete dephosphorylation of KaiC leads to dissociation of KaiA(2):KaiB(1), completing 1 cycle of the Kai oscillator (By similarity). Formation of the KaiB:KaiC complex is promoted by KaiA, helping switch KaiC from its autophosphorylation to autodephosphatase function (PubMed:24112939).[UniProtKB:Q79PF6]<ref>PMID:24112939</ref> Binds oxidized quinones via the N-terminal PsR domain, allowing it to sense redox changes and possibly mediate clock input.[UniProtKB:Q79PF6]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v2/1v2z_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v2z ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
KaiA, KaiB and KaiC constitute the circadian clock machinery in cyanobacteria, and KaiA activates kaiBC expression whereas KaiC represses it. Here we show that KaiA is composed of three functional domains, the N-terminal amplitude-amplifier domain, the central period-adjuster domain and the C-terminal clock-oscillator domain. The C-terminal domain is responsible for dimer formation, binding to KaiC, enhancing KaiC phosphorylation and generating the circadian oscillations. The X-ray crystal structure at a resolution of 1.8 A of the C-terminal clock-oscillator domain of KaiA from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 shows that residue His270, located at the center of a KaiA dimer concavity, is essential to KaiA function. KaiA binding to KaiC probably occurs via the concave surface. On the basis of the structure, we predict the structural roles of the residues that affect circadian oscillations.
-
'''Crystal structure of the C-terminal domain of Thermosynechococcus elongatus BP-1 KaiA'''
+
Crystal structure of the C-terminal clock-oscillator domain of the cyanobacterial KaiA protein.,Uzumaki T, Fujita M, Nakatsu T, Hayashi F, Shibata H, Itoh N, Kato H, Ishiura M Nat Struct Mol Biol. 2004 Jul;11(7):623-31. Epub 2004 May 30. PMID:15170179<ref>PMID:15170179</ref>
-
 
+
-
 
+
-
==Overview==
+
-
KaiA, KaiB and KaiC constitute the circadian clock machinery in cyanobacteria, and KaiA activates kaiBC expression whereas KaiC represses it. Here we show that KaiA is composed of three functional domains, the N-terminal amplitude-amplifier domain, the central period-adjuster domain and the C-terminal clock-oscillator domain. The C-terminal domain is responsible for dimer formation, binding to KaiC, enhancing KaiC phosphorylation and generating the circadian oscillations. The X-ray crystal structure at a resolution of 1.8 A of the C-terminal clock-oscillator domain of KaiA from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 shows that residue His270, located at the center of a KaiA dimer concavity, is essential to KaiA function. KaiA binding to KaiC probably occurs via the concave surface. On the basis of the structure, we predict the structural roles of the residues that affect circadian oscillations.
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
1V2Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2Z OCA].
+
</div>
 +
<div class="pdbe-citations 1v2z" style="background-color:#fffaf0;"></div>
-
==Reference==
+
==See Also==
-
Crystal structure of the C-terminal clock-oscillator domain of the cyanobacterial KaiA protein., Uzumaki T, Fujita M, Nakatsu T, Hayashi F, Shibata H, Itoh N, Kato H, Ishiura M, Nat Struct Mol Biol. 2004 Jul;11(7):623-31. Epub 2004 May 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15170179 15170179]
+
*[[Circadian clock protein 3D structures|Circadian clock protein 3D structures]]
-
[[Category: Bacteria]]
+
== References ==
-
[[Category: Single protein]]
+
<references/>
-
[[Category: Fujita, M.]]
+
__TOC__
-
[[Category: Hayashi, F.]]
+
</StructureSection>
-
[[Category: Ishiura, M.]]
+
[[Category: Large Structures]]
-
[[Category: Itoh, N.]]
+
[[Category: Thermosynechococcus vestitus BP-1]]
-
[[Category: Kato, H.]]
+
[[Category: Fujita M]]
-
[[Category: Nakatsu, T.]]
+
[[Category: Hayashi F]]
-
[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
+
[[Category: Ishiura M]]
-
[[Category: Shibata, H.]]
+
[[Category: Itoh N]]
-
[[Category: Uzumaki, T.]]
+
[[Category: Kato H]]
-
[[Category: All alpha]]
+
[[Category: Nakatsu T]]
-
[[Category: Riken structural genomics/proteomics initiative]]
+
[[Category: Shibata H]]
-
[[Category: Rsgi]]
+
[[Category: Uzumaki T]]
-
[[Category: Structural genomic]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:00:41 2008''
+

Current revision

Crystal structure of the C-terminal domain of Thermosynechococcus elongatus BP-1 KaiA

PDB ID 1v2z

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1v2z"
Personal tools