1vcm

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(New page: 200px<br /><applet load="1vcm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vcm, resolution 2.35&Aring;" /> '''Crystal Structure of...)
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[[Image:1vcm.gif|left|200px]]<br /><applet load="1vcm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1vcm, resolution 2.35&Aring;" />
 
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'''Crystal Structure of T.th. HB8 CTP synthetase'''<br />
 
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==Overview==
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==Crystal Structure of T.th. HB8 CTP synthetase==
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CTP synthetase (CTPs) catalyzes the last step in CTP biosynthesis, in, which ammonia generated at the glutaminase domain reacts with the, ATP-phosphorylated UTP at the synthetase domain to give CTP. Glutamine, hydrolysis is active in the presence of ATP and UTP and is stimulated by, the addition of GTP. We report the crystal structures of Thermus, thermophilus HB8 CTPs alone, CTPs with 3SO4(2-), and CTPs with glutamine., The enzyme is folded into a homotetramer with a cross-shaped structure., Based on the binding mode of sulfate anions to the synthetase site, ATP, and UTP are computer modeled into CTPs with a geometry favorable for the, reaction. Glutamine bound to the glutaminase domain is situated next to, the triad of Glu-His-Cys as a catalyst and a water molecule. Structural, information provides an insight into the conformational changes associated, with the binding of ATP and UTP and the formation of the GTP binding site.
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<StructureSection load='1vcm' size='340' side='right'caption='[[1vcm]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1vcm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VCM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VCM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vcm OCA], [https://pdbe.org/1vcm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vcm RCSB], [https://www.ebi.ac.uk/pdbsum/1vcm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vcm ProSAT], [https://www.topsan.org/Proteins/RSGI/1vcm TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PYRG_THET8 PYRG_THET8] Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vc/1vcm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vcm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CTP synthetase (CTPs) catalyzes the last step in CTP biosynthesis, in which ammonia generated at the glutaminase domain reacts with the ATP-phosphorylated UTP at the synthetase domain to give CTP. Glutamine hydrolysis is active in the presence of ATP and UTP and is stimulated by the addition of GTP. We report the crystal structures of Thermus thermophilus HB8 CTPs alone, CTPs with 3SO4(2-), and CTPs with glutamine. The enzyme is folded into a homotetramer with a cross-shaped structure. Based on the binding mode of sulfate anions to the synthetase site, ATP and UTP are computer modeled into CTPs with a geometry favorable for the reaction. Glutamine bound to the glutaminase domain is situated next to the triad of Glu-His-Cys as a catalyst and a water molecule. Structural information provides an insight into the conformational changes associated with the binding of ATP and UTP and the formation of the GTP binding site.
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==About this Structure==
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Crystal structures of CTP synthetase reveal ATP, UTP, and glutamine binding sites.,Goto M, Omi R, Nakagawa N, Miyahara I, Hirotsu K Structure. 2004 Aug;12(8):1413-23. PMID:15296735<ref>PMID:15296735</ref>
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1VCM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Active as [http://en.wikipedia.org/wiki/CTP_synthase CTP synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.2 6.3.4.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VCM OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of CTP synthetase reveal ATP, UTP, and glutamine binding sites., Goto M, Omi R, Nakagawa N, Miyahara I, Hirotsu K, Structure. 2004 Aug;12(8):1413-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15296735 15296735]
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</div>
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[[Category: CTP synthase]]
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<div class="pdbe-citations 1vcm" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Goto, M.]]
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[[Category: Goto M]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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[[Category: tetramer]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:35:13 2007''
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Current revision

Crystal Structure of T.th. HB8 CTP synthetase

PDB ID 1vcm

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