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2lef

From Proteopedia

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{{Seed}}
 
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[[Image:2lef.png|left|200px]]
 
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==LEF1 HMG DOMAIN (FROM MOUSE), COMPLEXED WITH DNA (15BP), NMR, 12 STRUCTURES==
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The line below this paragraph, containing "STRUCTURE_2lef", creates the "Structure Box" on the page.
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<StructureSection load='2lef' size='340' side='right'caption='[[2lef]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2lef]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1lef 1lef]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LEF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lef OCA], [https://pdbe.org/2lef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lef RCSB], [https://www.ebi.ac.uk/pdbsum/2lef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lef ProSAT]</span></td></tr>
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{{STRUCTURE_2lef| PDB=2lef | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEF1_MOUSE LEF1_MOUSE] Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (By similarity). Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1.<ref>PMID:11445543</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/le/2lef_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2lef ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lymphoid enhancer-binding factor (LEF-1) and the closely related T-cell factor 1 (TCF-1) are sequence-specific and cell-type-specific DNA-binding proteins that play important regulatory roles in organogenesis and thymocyte differentiation. LEF-1 participates in regulation of the enhancer associated with the T cell receptor (TCR)-alpha gene by inducing a sharp bend in the DNA and facilitating interactions between Ets-1, PEBP2-alpha, and ATF/CREB, transcription factors bound at sites flanking the LEF-1 site. It seems that LEF-1 plays an architectural role in the assembly and function of this regulatory nucleoprotein complex. LEF-1 recognizes a specific nucleotide sequence through a high-mobility-group (HMG) domain. Proteins containing HMG domains bind DNA in the minor groove, bend the double helix, and recognize four-way junctions and other irregular DNA structures. Here we report the solution structure of a complex of the LEF-1 HMG domain and adjacent basic region with its cognate DNA. The structure reveals the HMG domain bound in the widened minor groove of a markedly distorted and bent double helix. The basic region binds across the narrowed major groove and contributes to DNA recognition.
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===LEF1 HMG DOMAIN (FROM MOUSE), COMPLEXED WITH DNA (15BP), NMR, 12 STRUCTURES===
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Structural basis for DNA bending by the architectural transcription factor LEF-1.,Love JJ, Li X, Case DA, Giese K, Grosschedl R, Wright PE Nature. 1995 Aug 31;376(6543):791-5. PMID:7651541<ref>PMID:7651541</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_7651541}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2lef" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 7651541 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_7651541}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2LEF is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1lef 1lef]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEF OCA].
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==Reference==
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<ref group="xtra">PMID:7651541</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Case, D A.]]
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[[Category: Case DA]]
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[[Category: Li, X.]]
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[[Category: Li X]]
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[[Category: Love, J J.]]
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[[Category: Love JJ]]
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[[Category: Wright, P E.]]
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[[Category: Wright PE]]
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[[Category: Dna bending]]
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[[Category: Dna binding]]
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[[Category: Hmg]]
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[[Category: Lef1]]
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[[Category: Tcr-a]]
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[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:43:41 2009''
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Current revision

LEF1 HMG DOMAIN (FROM MOUSE), COMPLEXED WITH DNA (15BP), NMR, 12 STRUCTURES

PDB ID 2lef

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