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| | <StructureSection load='2ntx' size='340' side='right'caption='[[2ntx]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='2ntx' size='340' side='right'caption='[[2ntx]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ntx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ntx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTX FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2nty|2nty]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At3g24620 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ntx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntx OCA], [https://pdbe.org/2ntx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ntx RCSB], [https://www.ebi.ac.uk/pdbsum/2ntx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntx ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ntx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntx OCA], [https://pdbe.org/2ntx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ntx RCSB], [https://www.ebi.ac.uk/pdbsum/2ntx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ROGF8_ARATH ROGF8_ARATH]] Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. Active as homodimer.<ref>PMID:17218277</ref>
| + | [https://www.uniprot.org/uniprot/ROGF8_ARATH ROGF8_ARATH] Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. Active as homodimer.<ref>PMID:17218277</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Berken, A]] | + | [[Category: Berken A]] |
| - | [[Category: Fricke, I]] | + | [[Category: Fricke I]] |
| - | [[Category: Scrima, A]] | + | [[Category: Scrima A]] |
| - | [[Category: Thomas, C]] | + | [[Category: Thomas C]] |
| - | [[Category: Wittinghofer, A]] | + | [[Category: Wittinghofer A]] |
| - | [[Category: Dimer]]
| + | |
| - | [[Category: Guanine nucleotide exchange factor]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
ROGF8_ARATH Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. Active as homodimer.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Rho of plants (Rop) proteins belong to the superfamily of small GTP-binding (G) proteins and are vital regulators of signal transduction in plants. In order to become activated, Rop proteins need to exchange GDP for GTP, an intrinsically slow process catalyzed by guanine nucleotide exchange factors (GEFs). RopGEFs show no homology to animal RhoGEFs, and the catalytic mechanism remains elusive. GEF-catalysed nucleotide exchange proceeds via transient ternary and stable binary complexes. While a number of structural studies have analyzed binary nucleotide-free G protein-GEF complexes, very little is known about the ternary complexes. Here we report the X-ray structure of the catalytic PRONE domain of RopGEF8 from Arabidopsis thaliana, both alone and in a ternary complex with Rop4 and GDP. The features of the latter complex, a transient intermediate of the exchange reaction never directly observed before, suggest a common mechanism of catalyzed nucleotide exchange applicable to small G proteins in general.
Structural evidence for a common intermediate in small G protein-GEF reactions.,Thomas C, Fricke I, Scrima A, Berken A, Wittinghofer A Mol Cell. 2007 Jan 12;25(1):141-9. PMID:17218277[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Thomas C, Fricke I, Scrima A, Berken A, Wittinghofer A. Structural evidence for a common intermediate in small G protein-GEF reactions. Mol Cell. 2007 Jan 12;25(1):141-9. PMID:17218277 doi:http://dx.doi.org/10.1016/j.molcel.2006.11.023
- ↑ Thomas C, Fricke I, Scrima A, Berken A, Wittinghofer A. Structural evidence for a common intermediate in small G protein-GEF reactions. Mol Cell. 2007 Jan 12;25(1):141-9. PMID:17218277 doi:http://dx.doi.org/10.1016/j.molcel.2006.11.023
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