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2o0f

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[[Image:2o0f.jpg|left|200px]]
 
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==Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3==
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The line below this paragraph, containing "STRUCTURE_2o0f", creates the "Structure Box" on the page.
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<SX load='2o0f' size='340' side='right' viewer='molstar' caption='[[2o0f]], [[Resolution|resolution]] 15.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2o0f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O0F FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 15.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o0f OCA], [https://pdbe.org/2o0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o0f RCSB], [https://www.ebi.ac.uk/pdbsum/2o0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o0f ProSAT]</span></td></tr>
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{{STRUCTURE_2o0f| PDB=2o0f | SCENE= }}
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</table>
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== Function ==
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'''Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3'''
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[https://www.uniprot.org/uniprot/RF3_ECOLI RF3_ECOLI] Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.[HAMAP-Rule:MF_00072]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o0/2o0f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o0f ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
During translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal structure of E. coli RF3*GDP, which has a three-domain architecture strikingly similar to the structure of EF-Tu*GTP. Biochemical data on RF3 mutants show that a surface region involving domains II and III is important for distinct steps in the action cycle of RF3. Furthermore, we present a cryo-electron microscopy (cryo-EM) structure of the posttermination ribosome bound with RF3 in the GTP form. Our data show that RF3*GTP binding induces large conformational changes in the ribosome, which break the interactions of the class I RF with both the decoding center and the GTPase-associated center of the ribosome, apparently leading to the release of the class I RF.
During translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal structure of E. coli RF3*GDP, which has a three-domain architecture strikingly similar to the structure of EF-Tu*GTP. Biochemical data on RF3 mutants show that a surface region involving domains II and III is important for distinct steps in the action cycle of RF3. Furthermore, we present a cryo-electron microscopy (cryo-EM) structure of the posttermination ribosome bound with RF3 in the GTP form. Our data show that RF3*GTP binding induces large conformational changes in the ribosome, which break the interactions of the class I RF with both the decoding center and the GTPase-associated center of the ribosome, apparently leading to the release of the class I RF.
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==About this Structure==
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RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors.,Gao H, Zhou Z, Rawat U, Huang C, Bouakaz L, Wang C, Cheng Z, Liu Y, Zavialov A, Gursky R, Sanyal S, Ehrenberg M, Frank J, Song H Cell. 2007 Jun 1;129(5):929-41. PMID:17540173<ref>PMID:17540173</ref>
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2O0F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O0F OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors., Gao H, Zhou Z, Rawat U, Huang C, Bouakaz L, Wang C, Cheng Z, Liu Y, Zavialov A, Gursky R, Sanyal S, Ehrenberg M, Frank J, Song H, Cell. 2007 Jun 1;129(5):929-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17540173 17540173]
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</div>
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<div class="pdbe-citations 2o0f" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</SX>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bouakaz, L.]]
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[[Category: Bouakaz L]]
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[[Category: Ehrenberg, M.]]
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[[Category: Ehrenberg M]]
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[[Category: Frank, J.]]
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[[Category: Frank J]]
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[[Category: Gao, H.]]
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[[Category: Gao H]]
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[[Category: Gursky, R.]]
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[[Category: Gursky R]]
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[[Category: Huang, C.]]
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[[Category: Huang C]]
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[[Category: Liu, Y.]]
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[[Category: Liu Y]]
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[[Category: Rawat, U.]]
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[[Category: Rawat U]]
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[[Category: Sanyal, S.]]
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[[Category: Sanyal S]]
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[[Category: Song, H.]]
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[[Category: Song H]]
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[[Category: Wang, C.]]
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[[Category: Wang C]]
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[[Category: Zavialov, A.]]
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[[Category: Zavialov A]]
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[[Category: Zhou, Z.]]
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[[Category: Zhou Z]]
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[[Category: Cryo-em]]
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[[Category: Rf3]]
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[[Category: Ribosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:09:05 2008''
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Current revision

Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3

2o0f, resolution 15.50Å

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