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| | <StructureSection load='2ohj' size='340' side='right'caption='[[2ohj]], [[Resolution|resolution]] 2.26Å' scene=''> | | <StructureSection load='2ohj' size='340' side='right'caption='[[2ohj]], [[Resolution|resolution]] 2.26Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ohj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"methanobacterium_thermoautotrophicus"_(sic)_zeikus_and_wolfe_1972 "methanobacterium thermoautotrophicus" (sic) zeikus and wolfe 1972]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OHJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ohj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OHJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.26Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ohh|2ohh]], [[2ohi|2ohi]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fprA, fpaA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 "Methanobacterium thermoautotrophicus" (sic) Zeikus and Wolfe 1972])</td></tr> | + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ohj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ohj OCA], [https://pdbe.org/2ohj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ohj RCSB], [https://www.ebi.ac.uk/pdbsum/2ohj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ohj ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ohj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ohj OCA], [https://pdbe.org/2ohj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ohj RCSB], [https://www.ebi.ac.uk/pdbsum/2ohj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ohj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/FPRA_METTM FPRA_METTM]] Probably functions as an electron acceptor for a hydrogenase; however there is an uncharacterized intermediate between the hydrogenase and flavoprotein A.<ref>PMID:9660187</ref>
| + | [https://www.uniprot.org/uniprot/FPRA_METTM FPRA_METTM] Probably functions as an electron acceptor for a hydrogenase; however there is an uncharacterized intermediate between the hydrogenase and flavoprotein A.<ref>PMID:9660187</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ermler, U]] | + | [[Category: Methanothermobacter thermautotrophicus]] |
| - | [[Category: Seedorf, H]] | + | [[Category: Ermler U]] |
| - | [[Category: Warkentin, E]] | + | [[Category: Seedorf H]] |
| - | [[Category: Beta-lactamase like domain]] | + | [[Category: Warkentin E]] |
| - | [[Category: Flavodoxine like domain]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
FPRA_METTM Probably functions as an electron acceptor for a hydrogenase; however there is an uncharacterized intermediate between the hydrogenase and flavoprotein A.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The di-iron flavoprotein F(420)H(2) oxidase found in methanogenic Archaea catalyzes the four-electron reduction of O(2) to 2H(2)O with 2 mol of reduced coenzyme F(420)(7,8-dimethyl-8-hydroxy-5-deazariboflavin). We report here on crystal structures of the homotetrameric F(420)H(2) oxidase from Methanothermobacter marburgensis at resolutions of 2.25 A, 2.25 A and 1.7 A, respectively, from which an active reduced state, an inactive oxidized state and an active oxidized state could be extracted. As found in structurally related A-type flavoproteins, the active site is formed at the dimer interface, where the di-iron center of one monomer is juxtaposed to FMN of the other. In the active reduced state [Fe(II)Fe(II)FMNH(2)], the two irons are surrounded by four histidines, one aspartate, one glutamate and one bridging aspartate. The so-called switch loop is in a closed conformation, thus preventing F(420) binding. In the inactive oxidized state [Fe(III)FMN], the iron nearest to FMN has moved to two remote binding sites, and the switch loop is changed to an open conformation. In the active oxidized state [Fe(III)Fe(III)FMN], both irons are positioned as in the reduced state but the switch loop is found in the open conformation as in the inactive oxidized state. It is proposed that the redox-dependent conformational change of the switch loop ensures alternate complete four-electron O(2) reduction and redox center re-reduction. On the basis of the known Si-Si stereospecific hydride transfer, F(420)H(2) was modeled into the solvent-accessible pocket in front of FMN. The inactive oxidized state might provide the molecular basis for enzyme inactivation by long-term O(2) exposure observed in some members of the FprA family.
Structure of coenzyme F420H2 oxidase (FprA), a di-iron flavoprotein from methanogenic Archaea catalyzing the reduction of O2 to H2O.,Seedorf H, Hagemeier CH, Shima S, Thauer RK, Warkentin E, Ermler U FEBS J. 2007 Mar;274(6):1588-99. PMID:17480207[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wasserfallen A, Ragettli S, Jouanneau Y, Leisinger T. A family of flavoproteins in the domains Archaea and Bacteria. Eur J Biochem. 1998 Jun 1;254(2):325-32. PMID:9660187
- ↑ Seedorf H, Hagemeier CH, Shima S, Thauer RK, Warkentin E, Ermler U. Structure of coenzyme F420H2 oxidase (FprA), a di-iron flavoprotein from methanogenic Archaea catalyzing the reduction of O2 to H2O. FEBS J. 2007 Mar;274(6):1588-99. PMID:17480207 doi:10.1111/j.1742-4658.2007.05706.x
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