2olv

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(New page: 200px<br /><applet load="2olv" size="350" color="white" frame="true" align="right" spinBox="true" caption="2olv, resolution 2.800&Aring;" /> '''Structural Insight ...)
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[[Image:2olv.jpg|left|200px]]<br /><applet load="2olv" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2olv, resolution 2.800&Aring;" />
 
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'''Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex'''<br />
 
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==Overview==
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==Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex==
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Peptidoglycan glycosyltransferases (GTs) catalyze the polymerization step, of cell-wall biosynthesis, are membrane-bound, and are highly conserved, across all bacteria. Long considered the "holy grail" of antibiotic, research, they represent an essential and easily accessible drug target, for antibiotic-resistant bacteria, including methicillin-resistant, Staphylococcus aureus. We have determined the 2.8 angstrom structure of a, bifunctional cell-wall cross-linking enzyme, including its transpeptidase, and GT domains, both unliganded and complexed with the substrate analog, moenomycin. The peptidoglycan GTs adopt a fold distinct from those of, other GT classes. The structures give insight into critical features of, the catalytic mechanism and key interactions required for enzyme, inhibition.
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<StructureSection load='2olv' size='340' side='right'caption='[[2olv]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2olv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OLV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OLV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M0E:MOENOMYCIN'>M0E</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2olv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2olv OCA], [https://pdbe.org/2olv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2olv RCSB], [https://www.ebi.ac.uk/pdbsum/2olv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2olv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9R744_STAAU Q9R744_STAAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/2olv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2olv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Peptidoglycan glycosyltransferases (GTs) catalyze the polymerization step of cell-wall biosynthesis, are membrane-bound, and are highly conserved across all bacteria. Long considered the "holy grail" of antibiotic research, they represent an essential and easily accessible drug target for antibiotic-resistant bacteria, including methicillin-resistant Staphylococcus aureus. We have determined the 2.8 angstrom structure of a bifunctional cell-wall cross-linking enzyme, including its transpeptidase and GT domains, both unliganded and complexed with the substrate analog moenomycin. The peptidoglycan GTs adopt a fold distinct from those of other GT classes. The structures give insight into critical features of the catalytic mechanism and key interactions required for enzyme inhibition.
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==About this Structure==
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Structural insight into the transglycosylation step of bacterial cell-wall biosynthesis.,Lovering AL, de Castro LH, Lim D, Strynadka NC Science. 2007 Mar 9;315(5817):1402-5. PMID:17347437<ref>PMID:17347437</ref>
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2OLV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=M0E:'>M0E</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OLV OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural insight into the transglycosylation step of bacterial cell-wall biosynthesis., Lovering AL, de Castro LH, Lim D, Strynadka NC, Science. 2007 Mar 9;315(5817):1402-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17347437 17347437]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2olv" style="background-color:#fffaf0;"></div>
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[[Category: Staphylococcus aureus]]
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[[Category: Castro, L.De.]]
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[[Category: Lim, D.]]
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[[Category: Lovering, A.L.]]
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[[Category: Strynadka, N.C.J.]]
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[[Category: M0E]]
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[[Category: glycosyltransferase family 51]]
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[[Category: lysozyme fold]]
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[[Category: transpeptidase fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:13:10 2008''
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==See Also==
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*[[Penicillin-binding protein 3D structures|Penicillin-binding protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Staphylococcus aureus]]
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[[Category: De Castro L]]
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[[Category: Lim D]]
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[[Category: Lovering AL]]
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[[Category: Strynadka NCJ]]

Current revision

Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex

PDB ID 2olv

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