2ooe

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(New page: 200px<br /><applet load="2ooe" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ooe, resolution 3.00&Aring;" /> '''Crystal structure of...)
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[[Image:2ooe.gif|left|200px]]<br /><applet load="2ooe" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ooe, resolution 3.00&Aring;" />
 
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'''Crystal structure of HAT domain of murine CstF-77'''<br />
 
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==Overview==
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==Crystal structure of HAT domain of murine CstF-77==
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Cleavage stimulation factor (CstF) is a heterotrimeric protein complex, essential for polyadenylation of mRNA precursors. The 77 kDa subunit, CstF-77, is known to mediate interactions with the other two subunits of, CstF as well as with other components of the polyadenylation machinery. We, report here the crystal structure of the HAT (half a TPR) domain of murine, CstF-77, as well as its C-terminal subdomain. Structural and biochemical, studies show that the HAT domain consists of two subdomains, HAT-N and, HAT-C domains, with drastically different orientations of their helical, motifs. The structures reveal a highly elongated dimer, spanning 165 A, with the dimerization mediated by the HAT-C domain. Light-scattering, studies, yeast two-hybrid assays, and analytical ultracentrifugation, measurements confirm this self-association. The mode of dimerization and, the relative arrangement of the HAT-N and HAT-C domains are unique to, CstF-77. Our data support a role for CstF dimerization in pre-mRNA 3' end, processing.
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<StructureSection load='2ooe' size='340' side='right'caption='[[2ooe]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ooe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OOE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ooe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ooe OCA], [https://pdbe.org/2ooe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ooe RCSB], [https://www.ebi.ac.uk/pdbsum/2ooe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ooe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CSTF3_MOUSE CSTF3_MOUSE] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oo/2ooe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ooe ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cleavage stimulation factor (CstF) is a heterotrimeric protein complex essential for polyadenylation of mRNA precursors. The 77 kDa subunit, CstF-77, is known to mediate interactions with the other two subunits of CstF as well as with other components of the polyadenylation machinery. We report here the crystal structure of the HAT (half a TPR) domain of murine CstF-77, as well as its C-terminal subdomain. Structural and biochemical studies show that the HAT domain consists of two subdomains, HAT-N and HAT-C domains, with drastically different orientations of their helical motifs. The structures reveal a highly elongated dimer, spanning 165 A, with the dimerization mediated by the HAT-C domain. Light-scattering studies, yeast two-hybrid assays, and analytical ultracentrifugation measurements confirm this self-association. The mode of dimerization and the relative arrangement of the HAT-N and HAT-C domains are unique to CstF-77. Our data support a role for CstF dimerization in pre-mRNA 3' end processing.
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==About this Structure==
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Crystal structure of murine CstF-77: dimeric association and implications for polyadenylation of mRNA precursors.,Bai Y, Auperin TC, Chou CY, Chang GG, Manley JL, Tong L Mol Cell. 2007 Mar 23;25(6):863-75. PMID:17386263<ref>PMID:17386263</ref>
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2OOE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OOE OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of murine CstF-77: dimeric association and implications for polyadenylation of mRNA precursors., Bai Y, Auperin TC, Chou CY, Chang GG, Manley JL, Tong L, Mol Cell. 2007 Mar 23;25(6):863-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17386263 17386263]
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</div>
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<div class="pdbe-citations 2ooe" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Auperin TC]]
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[[Category: Auperin, T.C.]]
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[[Category: Bai Y]]
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[[Category: Bai, Y.]]
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[[Category: Chang G-G]]
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[[Category: Chang, G.G.]]
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[[Category: Chou C-Y]]
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[[Category: Chou, C.Y.]]
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[[Category: Manley JL]]
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[[Category: Manley, J.L.]]
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[[Category: Tong L]]
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[[Category: Tong, L.]]
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[[Category: hat domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:14:28 2007''
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Current revision

Crystal structure of HAT domain of murine CstF-77

PDB ID 2ooe

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