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3f10

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Current revision

Crystal structure of Clostridium Acetobutylicum 8-oxoguanine DNA glycosylase in complex with 8-oxoguanosine

Structural highlights

3f10 is a 1 chain structure with sequence from Clostridium acetobutylicum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q97FM4_CLOAB

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

DNA is subject to a multitude of oxidative damages generated by oxidizing agents from metabolism and exogenous sources and by ionizing radiation. Guanine is particularly vulnerable to oxidation, and the most common oxidative product 8-oxoguanine (8-oxoG) is the most prevalent lesion observed in DNA molecules. 8-OxoG can form a normal Watson-Crick pair with cytosine (8-oxoG:C), but it can also form a stable Hoogsteen pair with adenine (8-oxoG:A), leading to a G:C-->T:A transversion after replication. Fortunately, 8-oxoG is recognized and excised by either of two DNA glycosylases of the base excision repair pathway: formamidopyrimidine-DNA glycosylase and 8-oxoguanine DNA glycosylase (Ogg). While Clostridium acetobutylicum Ogg (CacOgg) DNA glycosylase can specifically recognize and remove 8-oxoG, it displays little preference for the base opposite the lesion, which is unusual for a member of the Ogg1 family. This work describes the crystal structures of CacOgg in its apo form and in complex with 8-oxo-2'-deoxyguanosine. A structural comparison between the apo form and the liganded form of the enzyme reveals a structural reorganization of the C-terminal domain upon binding of 8-oxoG, similar to that reported for human OGG1. A structural comparison of CacOgg with human OGG1, in complex with 8-oxoG containing DNA, provides a structural rationale for the lack of opposite base specificity displayed by CacOgg.

Structural characterization of Clostridium acetobutylicum 8-oxoguanine DNA glycosylase in its apo form and in complex with 8-oxodeoxyguanosine.,Faucher F, Robey-Bond SM, Wallace SS, Doublie S J Mol Biol. 2009 Apr 3;387(3):669-79. Epub 2009 Feb 9. PMID:19361427^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Faucher F, Robey-Bond SM, Wallace SS, Doublie S. Structural characterization of Clostridium acetobutylicum 8-oxoguanine DNA glycosylase in its apo form and in complex with 8-oxodeoxyguanosine. J Mol Biol. 2009 Apr 3;387(3):669-79. Epub 2009 Feb 9. PMID:19361427 doi:10.1016/j.jmb.2009.01.067

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3f10"

Categories: Clostridium acetobutylicum | Large Structures | Doublie S | Faucher F

@@ Line 1: / Line 1: @@
 ==Crystal structure of Clostridium Acetobutylicum 8-oxoguanine DNA glycosylase in complex with 8-oxoguanosine==
-<StructureSection load='3f10' size='340' side='right' caption='[[3f10]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='3f10' size='340' side='right'caption='[[3f10]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3f10]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_acetobutyricum"_prevot_1940 "clostridium acetobutyricum" prevot 1940]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F10 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F10 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3f10]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_acetobutylicum Clostridium acetobutylicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F10 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F10 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8HG:2-DEOXY-8-OXOGUANOSINE'>8HG</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3f0z|3f0z]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8HG:2-DEOXY-8-OXOGUANOSINE'>8HG</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CAC2707, CA_C2707 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1488 "Clostridium acetobutyricum" Prevot 1940])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f10 OCA], [https://pdbe.org/3f10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f10 RCSB], [https://www.ebi.ac.uk/pdbsum/3f10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f10 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f10 OCA], [http://pdbe.org/3f10 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f10 RCSB], [http://www.ebi.ac.uk/pdbsum/3f10 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f10 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q97FM4_CLOAB Q97FM4_CLOAB]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 31: @@
 ==See Also==
-*[[DNA glycosylase|DNA glycosylase]]
+*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Clostridium acetobutyricum prevot 1940]]
+[[Category: Clostridium acetobutylicum]]
-[[Category: Doublie, S]]
+[[Category: Large Structures]]
-[[Category: Faucher, F]]
+[[Category: Doublie S]]
-[[Category: 8-oxoguanine]]
+[[Category: Faucher F]]
-[[Category: Glycosylase]]
-[[Category: Guanine]]
-[[Category: Hydrolase]]
-[[Category: Lyase]]
-[[Category: Ogg]]

3f10

From Proteopedia

Current revision

Crystal structure of Clostridium Acetobutylicum 8-oxoguanine DNA glycosylase in complex with 8-oxoguanosine

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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