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3f1i

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[[Image:3f1i.png|left|200px]]
 
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{{STRUCTURE_3f1i| PDB=3f1i | SCENE= }}
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==Human ESCRT-0 Core Complex==
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<StructureSection load='3f1i' size='340' side='right'caption='[[3f1i]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3f1i]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F1I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f1i OCA], [https://pdbe.org/3f1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f1i RCSB], [https://www.ebi.ac.uk/pdbsum/3f1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f1i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HGS_HUMAN HGS_HUMAN] Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f1/3f1i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f1i ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The human Hrs and STAM proteins comprise the ESCRT-0 complex, which sorts ubiquitinated cell surface receptors to lysosomes for degradation. Here we report a model for the complete ESCRT-0 complex based on the crystal structure of the Hrs-STAM core complex, previously solved domain structures, hydrodynamic measurements, and Monte Carlo simulations. ESCRT-0 expressed in insect cells has a hydrodynamic radius of RH = 7.9 nm and is a 1:1 heterodimer. The 2.3 Angstroms crystal structure of the ESCRT-0 core complex reveals two domain-swapped GAT domains and an antiparallel two-stranded coiled-coil, similar to yeast ESCRT-0. ESCRT-0 typifies a class of biomolecular assemblies that combine structured and unstructured elements, and have dynamic and open conformations to ensure versatility in target recognition. Coarse-grained Monte Carlo simulations constrained by experimental RH values for ESCRT-0 reveal a dynamic ensemble of conformations well suited for diverse functions.
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===Human ESCRT-0 Core Complex===
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Hybrid structural model of the complete human ESCRT-0 complex.,Ren X, Kloer DP, Kim YC, Ghirlando R, Saidi LF, Hummer G, Hurley JH Structure. 2009 Mar 11;17(3):406-16. PMID:19278655<ref>PMID:19278655</ref>
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{{ABSTRACT_PUBMED_19278655}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3f1i" style="background-color:#fffaf0;"></div>
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[[3f1i]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F1I OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019278655</ref><references group="xtra"/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Ghirlando, R.]]
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[[Category: Large Structures]]
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[[Category: Hummer, G.]]
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[[Category: Ghirlando R]]
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[[Category: Hurley, J H.]]
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[[Category: Hummer G]]
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[[Category: Kim, Y.]]
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[[Category: Hurley JH]]
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[[Category: Kloer, D P.]]
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[[Category: Kim Y]]
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[[Category: Ren, X.]]
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[[Category: Kloer DP]]
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[[Category: Saidi, L.]]
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[[Category: Ren X]]
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[[Category: Endosome]]
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[[Category: Saidi L]]
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[[Category: Escrt]]
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[[Category: Hg]]
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[[Category: Membrane]]
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[[Category: Metal-binding]]
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[[Category: Mvb]]
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[[Category: Phosphoprotein]]
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[[Category: Protein binding]]
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[[Category: Protein transport]]
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[[Category: Sh3 domain]]
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[[Category: Stam]]
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[[Category: Transport]]
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[[Category: Ubiquitin]]
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[[Category: Zinc-finger]]
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Current revision

Human ESCRT-0 Core Complex

PDB ID 3f1i

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