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3no7

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{{STRUCTURE_3no7| PDB=3no7 | SCENE= }}
 
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===Crystal structure of the centromere-binding protein ParB from plasmid pCXC100===
 
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{{ABSTRACT_PUBMED_21123191}}
 
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==About this Structure==
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==Crystal structure of the centromere-binding protein ParB from plasmid pCXC100==
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[[3no7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33973 Atcc 33973]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NO7 OCA].
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<StructureSection load='3no7' size='340' side='right'caption='[[3no7]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3no7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leifsonia_xyli_subsp._cynodontis Leifsonia xyli subsp. cynodontis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NO7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3no7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3no7 OCA], [https://pdbe.org/3no7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3no7 RCSB], [https://www.ebi.ac.uk/pdbsum/3no7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3no7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6EEF9_LEIXC Q6EEF9_LEIXC]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Plasmid pCXC100 from the Gram-positive bacterium Leifsonia xyli subsp. cynodontis uses a type Ib partition system that includes a centromere region, a Walker-type ATPase ParA and a centromere-binding protein ParB for stable segregation. However, ParB shows no detectable sequence homology to any DNA-binding motif. Here, we study the ParB centromere interaction by structural and biochemical approaches. The crystal structure of the C-terminal DNA-binding domain of ParB at 1.4 A resolution reveals a dimeric ribbon-helix-helix (RHH) motif, supporting the prevalence of RHH motif in centromere binding. Using hydroxyl radical footprinting and quantitative binding assays, we show that the centromere core comprises nine uninterrupted 9-nt direct repeats that can be successively bound by ParB dimers in a cooperative manner. However, the interaction of ParB with a single subsite requires 18 base pairs covering one immediate repeat as well as two halves of flanking repeats. Through mutagenesis, sequence specificity was determined for each position of an 18-bp subsite. These data suggest an unique centromere recognition mechanism by which the repeat sequence is jointly specified by adjacent ParB dimers bound to an overlapped region.
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==Reference==
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Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.,Huang L, Yin P, Zhu X, Zhang Y, Ye K Nucleic Acids Res. 2010 Dec 1. PMID:21123191<ref>PMID:21123191</ref>
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<ref group="xtra">PMID:021123191</ref><references group="xtra"/><references/>
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[[Category: Atcc 33973]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Ye, K.]]
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</div>
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[[Category: Dna binding protein]]
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<div class="pdbe-citations 3no7" style="background-color:#fffaf0;"></div>
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[[Category: Ribbon-helix-helix]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Leifsonia xyli subsp. cynodontis]]
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[[Category: Ye K]]

Current revision

Crystal structure of the centromere-binding protein ParB from plasmid pCXC100

PDB ID 3no7

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