3nqw

From Proteopedia

(Difference between revisions)

Current revision

A metazoan ortholog of SpoT hydrolyzes ppGpp and plays a role in starvation responses

Structural highlights

3nqw is a 2 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.9Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MESH1_DROME ppGpp hydrolyzing enzyme involved in starvation response.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In nutrient-starved bacteria, RelA and SpoT proteins have key roles in reducing cell growth and overcoming stresses. Here we identify functional SpoT orthologs in metazoa (named Mesh1, encoded by HDDC3 in human and Q9VAM9 in Drosophila melanogaster) and reveal their structures and functions. Like the bacterial enzyme, Mesh1 proteins contain an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn(2+) binding. Consistent with these structural data, Mesh1 efficiently catalyzes hydrolysis of guanosine 3',5'-diphosphate (ppGpp) both in vitro and in vivo. Mesh1 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria. Notably, deletion of Mesh1 (Q9VAM9) in Drosophila induces retarded body growth and impaired starvation resistance. Microarray analyses reveal that the amino acid-starved Mesh1 null mutant has highly downregulated DNA and protein synthesis-related genes and upregulated stress-responsible genes. These data suggest that metazoan SpoT orthologs have an evolutionarily conserved function in starvation responses.

A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses.,Sun D, Lee G, Lee JH, Kim HY, Rhee HW, Park SY, Kim KJ, Kim Y, Kim BY, Hong JI, Park C, Choy HE, Kim JH, Jeon YH, Chung J Nat Struct Mol Biol. 2010 Oct;17(10):1188-94. Epub 2010 Sep 5. PMID:20818390^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sun D, Lee G, Lee JH, Kim HY, Rhee HW, Park SY, Kim KJ, Kim Y, Kim BY, Hong JI, Park C, Choy HE, Kim JH, Jeon YH, Chung J. A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses. Nat Struct Mol Biol. 2010 Oct;17(10):1188-94. Epub 2010 Sep 5. PMID:20818390 doi:http://dx.doi.org/10.1038/nsmb.1906

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nqw"

@@ Line 1: / Line 1: @@
 ==A metazoan ortholog of SpoT hydrolyzes ppGpp and plays a role in starvation responses==
-<StructureSection load='3nqw' size='340' side='right' caption='[[3nqw]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+<StructureSection load='3nqw' size='340' side='right'caption='[[3nqw]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nqw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NQW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nqw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NQW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG11900, Dmel_CG11900 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nqw OCA], [http://pdbe.org/3nqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nqw RCSB], [http://www.ebi.ac.uk/pdbsum/3nqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nqw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nqw OCA], [https://pdbe.org/3nqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nqw RCSB], [https://www.ebi.ac.uk/pdbsum/3nqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nqw ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MESH1_DROME MESH1_DROME]] ppGpp hydrolyzing enzyme involved in starvation response.
+[https://www.uniprot.org/uniprot/MESH1_DROME MESH1_DROME] ppGpp hydrolyzing enzyme involved in starvation response.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nq/3nqw_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nq/3nqw_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 33: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
-[[Category: Chung, J]]
+[[Category: Large Structures]]
-[[Category: Jeon, Y H]]
+[[Category: Chung J]]
-[[Category: Kim, H Y]]
+[[Category: Jeon YH]]
-[[Category: Kim, K J]]
+[[Category: Kim HY]]
-[[Category: Sun, D W]]
+[[Category: Kim KJ]]
-[[Category: Hydrolase]]
+[[Category: Sun DW]]
-[[Category: Ppgpp hydrolase]]
-[[Category: Pyrophosphohydrolase]]
-[[Category: Stringent response]]

3nqw

From Proteopedia

Current revision

A metazoan ortholog of SpoT hydrolyzes ppGpp and plays a role in starvation responses

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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