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3nrq

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[[Image:3nrq.png|left|200px]]
 
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==Crystal structure of copper-reconstituted FetP from uropathogenic Escherichia coli strain F11==
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The line below this paragraph, containing "STRUCTURE_3nrq", creates the "Structure Box" on the page.
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<StructureSection load='3nrq' size='340' side='right'caption='[[3nrq]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nrq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_F11 Escherichia coli F11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NRQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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{{STRUCTURE_3nrq| PDB=3nrq | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nrq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nrq OCA], [https://pdbe.org/3nrq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nrq RCSB], [https://www.ebi.ac.uk/pdbsum/3nrq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nrq ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the uropathogenic Escherichia coli strain F11, in silico genome analysis revealed the dicistronic iron uptake operon fetMP, which is under iron-regulated control mediated by the Fur regulator. The expression of fetMP in a mutant strain lacking known iron uptake systems improved growth under iron depletion and increased cellular iron accumulation. FetM is a member of the iron/lead transporter superfamily and is essential for iron uptake by the Fet system. FetP is a periplasmic protein that enhanced iron uptake by FetM. Recombinant FetP bound Cu(II) and the iron analog Mn(II) at distinct sites. The crystal structure of the FetP dimer reveals a copper site in each FetP subunit that adopts two conformations: CuA with a tetrahedral geometry composed of His(44), Met(90), His(97), and His(127), and CuB, a second degenerate octahedral geometry with the addition of Glu(46). The copper ions of each site occupy distinct positions and are separated by approximately 1.3 A. Nearby, a putative additional Cu(I) binding site is proposed as an electron source that may function with CuA/CuB displacement to reduce Fe(III) for transport by FetM. Together, these data indicate that FetMP is an additional iron uptake system composed of a putative iron permease and an iron-scavenging and potentially iron-reducing periplasmic protein.
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===Crystal structure of copper-reconstituted FetP from uropathogenic Escherichia coli strain F11===
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Characterization of a Dipartite Iron Uptake System from Uropathogenic Escherichia coli Strain F11.,Koch D, Chan AC, Murphy ME, Lilie H, Grass G, Nies DH J Biol Chem. 2011 Jul 15;286(28):25317-30. Epub 2011 May 19. PMID:21596746<ref>PMID:21596746</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3nrq" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21596746 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21596746}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli F11]]
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[[3nrq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NRQ OCA].
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[[Category: Large Structures]]
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[[Category: Chan ACK]]
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==Reference==
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[[Category: Murphy MEP]]
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<ref group="xtra">PMID:021596746</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
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[[Category: Chan, A C.K.]]
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[[Category: Murphy, M E.P.]]
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[[Category: Copper binding]]
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[[Category: Immunoglobulin-like fold]]
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[[Category: Iron transporter]]
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[[Category: Transport protein]]
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Current revision

Crystal structure of copper-reconstituted FetP from uropathogenic Escherichia coli strain F11

PDB ID 3nrq

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