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3ns4

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{{Seed}}
 
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[[Image:3ns4.jpg|left|200px]]
 
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==Structure of a C-terminal fragment of its Vps53 subunit suggests similarity of GARP to a family of tethering complexes==
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The line below this paragraph, containing "STRUCTURE_3ns4", creates the "Structure Box" on the page.
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<StructureSection load='3ns4' size='340' side='right'caption='[[3ns4]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ns4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NS4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3ns4| PDB=3ns4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ns4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ns4 OCA], [https://pdbe.org/3ns4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ns4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ns4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ns4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VPS53_YEAST VPS53_YEAST] Involved in retrograde transport from early and late endosomes to late Golgi by linking the vesicle through the t-SNARE TGL1 to the Golgi, leading to the membrane fusion between late Golgi and endosomal vesicles.<ref>PMID:10637310</ref> <ref>PMID:11689439</ref> <ref>PMID:12377769</ref> <ref>PMID:12446664</ref> <ref>PMID:12686613</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/3ns4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ns4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Golgi-associated retrograde protein (GARP) complex is a membrane-tethering complex that functions in traffic from endosomes to the trans-Golgi network. Here we present the structure of a C-terminal fragment of the Vps53 subunit, important for binding endosome-derived vesicles, at a resolution of 2.9 A. We show that the C terminus consists of two alpha-helical bundles arranged in tandem, and we identify a highly conserved surface patch, which may play a role in vesicle recognition. Mutations of the surface result in defects in membrane traffic. The fold of the Vps53 C terminus is strongly reminiscent of proteins that belong to three other tethering complexes--Dsl1, conserved oligomeric Golgi, and the exocyst--thought to share a common evolutionary origin. Thus, the structure of the Vps53 C terminus suggests that GARP belongs to this family of complexes.
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===Structure of a C-terminal fragment of its Vps53 subunit suggests similarity of GARP to a family of tethering complexes===
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Structure of a C-terminal fragment of its Vps53 subunit suggests similarity of Golgi-associated retrograde protein (GARP) complex to a family of tethering complexes.,Vasan N, Hutagalung A, Novick P, Reinisch KM Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14176-81. Epub 2010 Jul 26. PMID:20660722<ref>PMID:20660722</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ns4" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20660722}}, adds the Publication Abstract to the page
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*[[Vacuolar protein sorting-associated protein 3D structures|Vacuolar protein sorting-associated protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20660722 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20660722}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3NS4 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NS4 OCA].
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==Reference==
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<ref group="xtra">PMID:20660722</ref><references group="xtra"/>
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Reinisch, K M.]]
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[[Category: Reinisch KM]]
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[[Category: Vasan, N.]]
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[[Category: Vasan N]]
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[[Category: Arl1]]
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[[Category: Garp complex component]]
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[[Category: Helical bundle]]
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[[Category: Membrane tethering complex]]
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[[Category: Membrane traffic]]
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[[Category: Protein binding]]
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[[Category: Vps51]]
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[[Category: Vps52]]
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[[Category: Vps54]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 15 10:52:17 2010''
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Current revision

Structure of a C-terminal fragment of its Vps53 subunit suggests similarity of GARP to a family of tethering complexes

PDB ID 3ns4

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