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4pkx

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The structure of a conserved Piezo channel domain reveals a novel beta sandwich fold

Structural highlights

4pkx is a 1 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.54Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PIEZ1_CAEEL Pore-forming subunit of a mechanosensitive non-specific cation channel (By similarity). Generates currents characterized by a linear current-voltage relationship (By similarity). Plays a role in reproduction by positively regulating inter-tissue signaling to promote oocyte maturation, ovulation and fertilization, and sperm navigation from and to the spermatheca (PubMed:32490809). May play a role in regulating cytosolic and endoplasmic reticulum calcium ion release (PubMed:32490809).[UniProtKB:Q92508]^[1]

Publication Abstract from PubMed

Piezo has recently been identified as a family of eukaryotic mechanosensitive channels composed of subunits containing over 2,000 amino acids, without recognizable sequence similarity to other channels. Here, we present the crystal structure of a large, conserved extramembrane domain located just before the last predicted transmembrane helix of C. elegans PIEZO, which adopts a topologically distinct beta sandwich fold. The structure was also determined of a point mutation located on a conserved surface at the position equivalent to the human PIEZO1 mutation found in dehydrated hereditary stomatocytosis patients (M2225R). While the point mutation does not change the overall domain structure, it does alter the surface electrostatic potential that may perturb interactions with a yet-to-be-identified ligand or protein. The lack of structural similarity between this domain and any previously characterized fold, including those of eukaryotic and bacterial channels, highlights the distinctive nature of the Piezo family of eukaryotic mechanosensitive channels.

The Structure of a Conserved Piezo Channel Domain Reveals a Topologically Distinct beta Sandwich Fold.,Kamajaya A, Kaiser JT, Lee J, Reid M, Rees DC Structure. 2014 Sep 17. pii: S0969-2126(14)00254-8. doi:, 10.1016/j.str.2014.08.009. PMID:25242456^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bai X, Bouffard J, Lord A, Brugman K, Sternberg PW, Cram EJ, Golden A. Caenorhabditis elegans PIEZO channel coordinates multiple reproductive tissues to govern ovulation. Elife. 2020 Jun 3;9:e53603. doi: 10.7554/eLife.53603. PMID:32490809 doi:http://dx.doi.org/10.7554/eLife.53603
↑ Kamajaya A, Kaiser JT, Lee J, Reid M, Rees DC. The Structure of a Conserved Piezo Channel Domain Reveals a Topologically Distinct beta Sandwich Fold. Structure. 2014 Sep 17. pii: S0969-2126(14)00254-8. doi:, 10.1016/j.str.2014.08.009. PMID:25242456 doi:http://dx.doi.org/10.1016/j.str.2014.08.009

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4pkx"

@@ Line 3: / Line 3: @@
 <StructureSection load='4pkx' size='340' side='right'caption='[[4pkx]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4pkx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4pkx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PKX FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4pke|4pke]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.54&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C10C5.1, CELE_C10C5.1, T20D3.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkx OCA], [https://pdbe.org/4pkx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pkx RCSB], [https://www.ebi.ac.uk/pdbsum/4pkx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pkx ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkx OCA], [http://pdbe.org/4pkx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pkx RCSB], [http://www.ebi.ac.uk/pdbsum/4pkx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pkx ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PIEZ1_CAEEL PIEZ1_CAEEL] Pore-forming subunit of a mechanosensitive non-specific cation channel (By similarity). Generates currents characterized by a linear current-voltage relationship (By similarity). Plays a role in reproduction by positively regulating inter-tissue signaling to promote oocyte maturation, ovulation and fertilization, and sperm navigation from and to the spermatheca (PubMed:32490809). May play a role in regulating cytosolic and endoplasmic reticulum calcium ion release (PubMed:32490809).[UniProtKB:Q92508]<ref>PMID:32490809</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Caeel]]
+[[Category: Caenorhabditis elegans]]
 [[Category: Large Structures]]
-[[Category: Kaiser, J]]
+[[Category: Kaiser J]]
-[[Category: Kamajaya, A]]
+[[Category: Kamajaya A]]
-[[Category: Lee, J]]
+[[Category: Lee J]]
-[[Category: Rees, D C]]
+[[Category: Rees DC]]
-[[Category: Reid, M]]
+[[Category: Reid M]]
-[[Category: Channel]]
-[[Category: Mechanosensitive]]
-[[Category: Membrane protein]]
-[[Category: Piezo]]

4pkx

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Current revision

The structure of a conserved Piezo channel domain reveals a novel beta sandwich fold

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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