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| | ==Crystal structure of tRNA-dependent lantibiotic dehydratase NisB in complex with NisA leader peptide== | | ==Crystal structure of tRNA-dependent lantibiotic dehydratase NisB in complex with NisA leader peptide== |
| - | <StructureSection load='4wd9' size='340' side='right' caption='[[4wd9]], [[Resolution|resolution]] 2.90Å' scene=''> | + | <StructureSection load='4wd9' size='340' side='right'caption='[[4wd9]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wd9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"lactococcus_lactis_subsp._diacitilactis_(sic)"_(garvie_and_farrow_1982)_schleifer_et_al._1986 "lactococcus lactis subsp. diacitilactis (sic)" (garvie and farrow 1982) schleifer et al. 1986]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WD9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wd9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis Lactococcus lactis subsp. lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WD9 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nisB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1360 "Lactococcus lactis subsp. diacitilactis (sic)" (Garvie and Farrow 1982) Schleifer et al. 1986])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wd9 OCA], [http://pdbe.org/4wd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wd9 RCSB], [http://www.ebi.ac.uk/pdbsum/4wd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wd9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wd9 OCA], [https://pdbe.org/4wd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wd9 RCSB], [https://www.ebi.ac.uk/pdbsum/4wd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wd9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NISB_LACLL NISB_LACLL]] Involved in the post-translational modification of the lantibiotic nisin. | + | [https://www.uniprot.org/uniprot/NISB_LACLL NISB_LACLL] Involved in the post-translational modification of the lantibiotic nisin. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Hao, Y]] | + | [[Category: Lactococcus lactis subsp. lactis]] |
| - | [[Category: Nair, S K]] | + | [[Category: Large Structures]] |
| - | [[Category: Biosynthetic protein]] | + | [[Category: Hao Y]] |
| - | [[Category: Class i lantibiotic dehydratase]] | + | [[Category: Nair SK]] |
| Structural highlights
Function
NISB_LACLL Involved in the post-translational modification of the lantibiotic nisin.
Publication Abstract from PubMed
Lantibiotics are a class of peptide antibiotics that contain one or more thioether bonds. The lantibiotic nisin is an antimicrobial peptide that is widely used as a food preservative to combat food-borne pathogens. Nisin contains dehydroalanine and dehydrobutyrine residues that are formed by the dehydration of Ser/Thr by the lantibiotic dehydratase NisB (ref. 2). Recent biochemical studies revealed that NisB glutamylates Ser/Thr side chains as part of the dehydration process. However, the molecular mechanism by which NisB uses glutamate to catalyse dehydration remains unresolved. Here we show that this process involves glutamyl-tRNAGlu to activate Ser/Thr residues. In addition, the 2.9-A crystal structure of NisB in complex with its substrate peptide NisA reveals the presence of two separate domains that catalyse the Ser/Thr glutamylation and glutamate elimination steps. The co-crystal structure also provides insights into substrate recognition by lantibiotic dehydratases. Our findings demonstrate an unexpected role for aminoacyl-tRNA in the formation of dehydroamino acids in lantibiotics, and serve as a basis for the functional characterization of the many lantibiotic-like dehydratases involved in the biosynthesis of other classes of natural products.
Structure and mechanism of the tRNA-dependent lantibiotic dehydratase NisB.,Ortega MA, Hao Y, Zhang Q, Walker MC, van der Donk WA, Nair SK Nature. 2014 Oct 26. doi: 10.1038/nature13888. PMID:25363770[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ortega MA, Hao Y, Zhang Q, Walker MC, van der Donk WA, Nair SK. Structure and mechanism of the tRNA-dependent lantibiotic dehydratase NisB. Nature. 2014 Oct 26. doi: 10.1038/nature13888. PMID:25363770 doi:http://dx.doi.org/10.1038/nature13888
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