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5owp
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5owp is ON HOLD Authors: Bermejo, I.A., Albuquerque, I.S., Somovilla, V.J., Martinez-Saez, N., Castro-Lopez, J., Garcia-Martin, F., Hinou, H., Nishi...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of glycopeptide "GVTSAfPDT*RPAP" in complex with scFv-SM3== | |
| + | <StructureSection load='5owp' size='340' side='right'caption='[[5owp]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5owp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OWP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OWP FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4FB:(4S)-4-FLUORO-L-PROLINE'>4FB</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5owp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5owp OCA], [https://pdbe.org/5owp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5owp RCSB], [https://www.ebi.ac.uk/pdbsum/5owp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5owp ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LV1D_MOUSE LV1D_MOUSE] [https://www.uniprot.org/uniprot/HVM32_MOUSE HVM32_MOUSE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A structure-based design of a new generation of tumor-associated glycopeptides with improved affinity against two anti-MUC1 antibodies is described. These unique antigens feature a fluorinated proline residue, such as a (4S)-4-fluoro-l-proline or 4,4-difluoro-l-proline, at the most immunogenic domain. Binding assays using biolayer interferometry reveal 3-fold to 10-fold affinity improvement with respect to the natural (glyco)peptides. According to X-ray crystallography and MD simulations, the fluorinated residues stabilize the antigen-antibody complex by enhancing key CH/pi interactions. Interestingly, a notable improvement in detection of cancer-associated anti-MUC1 antibodies from serum of patients with prostate cancer is achieved with the non-natural antigens, which proves that these derivatives can be considered better diagnostic tools than the natural antigen for prostate cancer. | ||
| - | + | The Use of Fluoroproline in MUC1 Antigen Enables Efficient Detection of Antibodies in Patients with Prostate Cancer.,Somovilla VJ, Bermejo IA, Albuquerque IS, Martinez-Saez N, Castro-Lopez J, Garcia-Martin F, Companon I, Hinou H, Nishimura SI, Jimenez-Barbero J, Asensio JL, Avenoza A, Busto JH, Hurtado-Guerrero R, Peregrina JM, Bernardes GJL, Corzana F J Am Chem Soc. 2017 Dec 12. doi: 10.1021/jacs.7b09447. PMID:29166012<ref>PMID:29166012</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5owp" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Antibody 3D structures|Antibody 3D structures]] |
| - | [[Category: | + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] |
| - | [[Category: | + | *[[3D structures of non-human antibody|3D structures of non-human antibody]] |
| - | [[Category: | + | == References == |
| - | [[Category: Castro-Lopez | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Hurtado-Guerrero | + | [[Category: Mus musculus]] |
| - | [[Category: | + | [[Category: Synthetic construct]] |
| - | [[Category: | + | [[Category: Albuquerque IS]] |
| - | [[Category: | + | [[Category: Asensio JL]] |
| - | [[Category: | + | [[Category: Avenoza A]] |
| + | [[Category: Bermejo IA]] | ||
| + | [[Category: Bernardes GJ]] | ||
| + | [[Category: Busto JH]] | ||
| + | [[Category: Castro-Lopez J]] | ||
| + | [[Category: Corzana F]] | ||
| + | [[Category: Garcia-Martin F]] | ||
| + | [[Category: Hinou H]] | ||
| + | [[Category: Hurtado-Guerrero R]] | ||
| + | [[Category: Jimenez-Barbero J]] | ||
| + | [[Category: Martinez-Saez N]] | ||
| + | [[Category: Nishimura S]] | ||
| + | [[Category: Peregrina JM]] | ||
| + | [[Category: Somovilla VJ]] | ||
Current revision
Crystal structure of glycopeptide "GVTSAfPDT*RPAP" in complex with scFv-SM3
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Categories: Large Structures | Mus musculus | Synthetic construct | Albuquerque IS | Asensio JL | Avenoza A | Bermejo IA | Bernardes GJ | Busto JH | Castro-Lopez J | Corzana F | Garcia-Martin F | Hinou H | Hurtado-Guerrero R | Jimenez-Barbero J | Martinez-Saez N | Nishimura S | Peregrina JM | Somovilla VJ
