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5omu
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5omu is ON HOLD until Aug 01 2019 Authors: Mallinson, S.J.B., Johnson, C.W., Neidle, E.L., Beckham, G.T., McGeehan, J.E. Description: Crystal struc...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Amycolatopsis cytochrome P450 GcoA in complex with syringol== | |
| + | <StructureSection load='5omu' size='340' side='right'caption='[[5omu]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5omu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_sp._ATCC_39116 Amycolatopsis sp. ATCC 39116]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OMU FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3DM:2,6-DIMETHOXYPHENOL'>3DM</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5omu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5omu OCA], [https://pdbe.org/5omu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5omu RCSB], [https://www.ebi.ac.uk/pdbsum/5omu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5omu ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A076MY51_AMYME A0A076MY51_AMYME] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion. | ||
| - | + | A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion.,Mallinson SJB, Machovina MM, Silveira RL, Garcia-Borras M, Gallup N, Johnson CW, Allen MD, Skaf MS, Crowley MF, Neidle EL, Houk KN, Beckham GT, DuBois JL, McGeehan JE Nat Commun. 2018 Jun 27;9(1):2487. doi: 10.1038/s41467-018-04878-2. PMID:29950589<ref>PMID:29950589</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5omu" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]] |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Amycolatopsis sp. ATCC 39116]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Beckham GT]] | ||
| + | [[Category: Johnson CW]] | ||
| + | [[Category: Mallinson SJB]] | ||
| + | [[Category: McGeehan JE]] | ||
| + | [[Category: Neidle EL]] | ||
Current revision
Crystal structure of Amycolatopsis cytochrome P450 GcoA in complex with syringol
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