This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
8w7p
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Extracellular domain of a sensor histidine kinase== | |
| + | <StructureSection load='8w7p' size='340' side='right'caption='[[8w7p]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8w7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_sp._FPU-7 Paenibacillus sp. FPU-7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8W7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8W7P FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8w7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8w7p OCA], [https://pdbe.org/8w7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8w7p RCSB], [https://www.ebi.ac.uk/pdbsum/8w7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8w7p ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We have previously isolated the Gram-positive chitin-degrading bacterium Paenibacillus sp. str. FPU-7. This bacterium traps chitin disaccharide (GlcNAc)2 on its cell surface using two homologous solute-binding proteins, NagB1 and NagB2. Bacteria use histidine kinase of the two-component regulatory system as an extracellular environment sensor. In this study, we found that nagS, which encodes a histidine kinase, is located next to the nagB1 gene. Biochemical experiments revealed that the NagS sensor domain (NagS30-294) interacts with the NagB1-(GlcNAc)2 complex. However, proof of NagS30-294 interacting with NagB1 without (GlcNAc)2 is currently unavailable. In contrast to NagB1, no complex formation was observed between NagS30-294 and NagB2, even in the presence of (GlcNAc)2. The NagS30-294 crystal structure at 1.8 A resolution suggested that the canonical tandem-Per-Arnt-Sim (PAS) fold recognizes the NagB1-(GlcNAc)2 complex. This study provides insight into the recognition of chitin oligosaccharides by bacteria. | ||
| - | + | Characterization of the extracellular domain of sensor histidine kinase NagS from Paenibacillus sp. str. FPU-7: nagS interacts with oligosaccharide binding protein NagB1 in complexes with N, N'-diacetylchitobiose.,Itoh T, Ogawa T, Hibi T, Kimoto H Biosci Biotechnol Biochem. 2023 Dec 6:zbad173. doi: 10.1093/bbb/zbad173. PMID:38059852<ref>PMID:38059852</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8w7p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Paenibacillus sp. FPU-7]] | ||
| + | [[Category: Hibi T]] | ||
| + | [[Category: Itoh T]] | ||
| + | [[Category: Kimoto H]] | ||
| + | [[Category: Ogawa T]] | ||
Revision as of 10:28, 10 January 2024
Extracellular domain of a sensor histidine kinase
| |||||||||||
