This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4uy6

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal structure of Histidine and SAH bound Histidine-specific methyltransferase EgtD from Mycobacterium smegmatis

Structural highlights

4uy6 is a 1 chain structure with sequence from Mycolicibacterium smegmatis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.04Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EGTD_MYCS2 Catalyzes the methylations of histidine to form N-alpha,N-alpha,N-alpha-trimethyl-L-histidine (also known as hercynine). Histidine and alpha-N,N-dimethylhistidine are preferred substrates.^[1]

Publication Abstract from PubMed

EgtD is an S-adenosyl-l-methionine (SAM)-dependent histidine N,N,N-methyltransferase that catalyzes the formation of hercynine from histidine in the ergothioneine biosynthetic process of Mycobacterium smegmatis. Ergothioneine is a secreted antioxidant that protects mycobacterium from oxidative stress. Here, we present three crystal structures of EgtD in the apo form, the histidine-bound form, and the S-adenosyl-l-homocysteine (SAH)/histidine-bound form. The study revealed that EgtD consists of two distinct domains: a typical methyltransferase domain and a unique substrate binding domain. The histidine binding pocket of the substrate binding domain primarily recognizes the imidazole ring and carboxylate group of histidine rather than the amino group, explaining the high selectivity for histidine and/or (mono-, di-) methylated histidine as substrates. In addition, SAM binding to the MTase domain induced a conformational change in EgtD to facilitate the methyl transfer reaction. The structural analysis provides insights into the putative catalytic mechanism of EgtD in a processive trimethylation reaction.

Structural insights into the histidine trimethylation activity of EgtD from Mycobacterium smegmatis.,Jeong JH, Cha HJ, Ha SC, Rojviriya C, Kim YG Biochem Biophys Res Commun. 2014 Oct 3;452(4):1098-103. doi:, 10.1016/j.bbrc.2014.09.058. Epub 2014 Sep 22. PMID:25251321^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Seebeck FP. In vitro reconstitution of Mycobacterial ergothioneine biosynthesis. J Am Chem Soc. 2010 May 19;132(19):6632-3. doi: 10.1021/ja101721e. PMID:20420449 doi:http://dx.doi.org/10.1021/ja101721e
↑ Jeong JH, Cha HJ, Ha SC, Rojviriya C, Kim YG. Structural insights into the histidine trimethylation activity of EgtD from Mycobacterium smegmatis. Biochem Biophys Res Commun. 2014 Oct 3;452(4):1098-103. doi:, 10.1016/j.bbrc.2014.09.058. Epub 2014 Sep 22. PMID:25251321 doi:http://dx.doi.org/10.1016/j.bbrc.2014.09.058

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4uy6"

Categories: Large Structures | Mycolicibacterium smegmatis | Jeong JH | Kim YG

@@ Line 1: / Line 1: @@
 ==Crystal structure of Histidine and SAH bound Histidine-specific methyltransferase EgtD from Mycobacterium smegmatis==
-<StructureSection load='4uy6' size='340' side='right' caption='[[4uy6]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
+<StructureSection load='4uy6' size='340' side='right'caption='[[4uy6]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4uy6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UY6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4uy6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UY6 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uy5|4uy5]], [[4uy7|4uy7]], [[4uz0|4uz0]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uy6 OCA], [http://pdbe.org/4uy6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uy6 RCSB], [http://www.ebi.ac.uk/pdbsum/4uy6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uy6 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uy6 OCA], [https://pdbe.org/4uy6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uy6 RCSB], [https://www.ebi.ac.uk/pdbsum/4uy6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uy6 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/EGTD_MYCS2 EGTD_MYCS2]] Catalyzes the methylations of histidine to form N-alpha,N-alpha,N-alpha-trimethyl-L-histidine (also known as hercynine). Histidine and alpha-N,N-dimethylhistidine are preferred substrates.<ref>PMID:20420449</ref>
+[https://www.uniprot.org/uniprot/EGTD_MYCS2 EGTD_MYCS2] Catalyzes the methylations of histidine to form N-alpha,N-alpha,N-alpha-trimethyl-L-histidine (also known as hercynine). Histidine and alpha-N,N-dimethylhistidine are preferred substrates.<ref>PMID:20420449</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Jeong, J H]]
+[[Category: Large Structures]]
-[[Category: Kim, Y G]]
+[[Category: Mycolicibacterium smegmatis]]
-[[Category: Antioxidant]]
+[[Category: Jeong JH]]
-[[Category: Mycobacterium smegmati]]
+[[Category: Kim YG]]
-[[Category: S-adenosyl-l-methionine]]
-[[Category: Transferase]]

4uy6

From Proteopedia

Current revision

Crystal structure of Histidine and SAH bound Histidine-specific methyltransferase EgtD from Mycobacterium smegmatis

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox