4v3c
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The structure of alpha2,3-sialyltransferase variant 2 from Pasteurella dagmatis in complex with the donor product CMP== |
| + | <StructureSection load='4v3c' size='340' side='right'caption='[[4v3c]], [[Resolution|resolution]] 1.84Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4v3c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pasteurella_dagmatis Pasteurella dagmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V3C FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v3c OCA], [https://pdbe.org/4v3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v3c RCSB], [https://www.ebi.ac.uk/pdbsum/4v3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v3c ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/K9UUI6_9PAST K9UUI6_9PAST] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Structure-guided active-site redesign of a family GT-80 beta-D-galactoside sialyltransferase (from Pasteurella dagmatis) to change enzyme regioselectivity from alpha-2,3 in the wild type to alpha-2,6 in a P7H-M117A double mutant is reported. Biochemical data for sialylation of lactose together with protein crystal structures demonstrate highly precise enzyme engineering. | ||
| - | + | Complete switch from alpha-2,3- to alpha-2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign.,Schmolzer K, Czabany T, Luley-Goedl C, Pavkov-Keller T, Ribitsch D, Schwab H, Gruber K, Weber H, Nidetzky B Chem Commun (Camb). 2015 Feb 21;51(15):3083-6. doi: 10.1039/c4cc09772f. PMID:25619424<ref>PMID:25619424</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4v3c" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Sialyltransferase 3D structures|Sialyltransferase 3D structures]] |
| - | [[Category: Pavkov-Keller | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Schwab | + | </StructureSection> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pasteurella dagmatis]] | ||
| + | [[Category: Czabany T]] | ||
| + | [[Category: Gruber K]] | ||
| + | [[Category: Luley-Goedl C]] | ||
| + | [[Category: Nidetzky B]] | ||
| + | [[Category: Pavkov-Keller T]] | ||
| + | [[Category: Ribitsch D]] | ||
| + | [[Category: Schmoelzer K]] | ||
| + | [[Category: Schwab H]] | ||
Current revision
The structure of alpha2,3-sialyltransferase variant 2 from Pasteurella dagmatis in complex with the donor product CMP
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