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4x6k
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(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4x6k is ON HOLD Authors: Owen, C.D., Tailford, L.E., Taylor, G.L., Juge, N. Description: Crystal structure of the intramolecular trans-sialidase fr...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the intramolecular trans-sialidase from Ruminococcus gnavus in complex with Siastatin B== | |
| + | <StructureSection load='4x6k' size='340' side='right'caption='[[4x6k]], [[Resolution|resolution]] 1.94Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4x6k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_gnavus_CC55_001C Ruminococcus gnavus CC55_001C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X6K FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3XR:(2S,3R,4S,5S)-2-(ACETYLAMINO)-5-CARBOXY-3,4-DIHYDROXYPIPERIDINIUM'>3XR</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x6k OCA], [https://pdbe.org/4x6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x6k RCSB], [https://www.ebi.ac.uk/pdbsum/4x6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x6k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The gastrointestinal mucus layer is colonized by a dense community of microbes catabolizing dietary and host carbohydrates during their expansion in the gut. Alterations in mucosal carbohydrate availability impact on the composition of microbial species. Ruminococcus gnavus is a commensal anaerobe present in the gastrointestinal tract of >90% of humans and overrepresented in inflammatory bowel diseases (IBD). Using a combination of genomics, enzymology and crystallography, we show that the mucin-degrader R. gnavus ATCC 29149 strain produces an intramolecular trans-sialidase (IT-sialidase) that cleaves off terminal alpha2-3-linked sialic acid from glycoproteins, releasing 2,7-anhydro-Neu5Ac instead of sialic acid. Evidence of IT-sialidases in human metagenomes indicates that this enzyme occurs in healthy subjects but is more prevalent in IBD metagenomes. Our results uncover a previously unrecognized enzymatic activity in the gut microbiota, which may contribute to the adaptation of intestinal bacteria to the mucosal environment in health and disease. | ||
| - | + | Discovery of intramolecular trans-sialidases in human gut microbiota suggests novel mechanisms of mucosal adaptation.,Tailford LE, Owen CD, Walshaw J, Crost EH, Hardy-Goddard J, Le Gall G, de Vos WM, Taylor GL, Juge N Nat Commun. 2015 Jul 8;6:7624. doi: 10.1038/ncomms8624. PMID:26154892<ref>PMID:26154892</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4x6k" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Neuraminidase 3D structures|Neuraminidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Juge N]] | ||
| + | [[Category: Owen CD]] | ||
| + | [[Category: Tailford LE]] | ||
| + | [[Category: Taylor GL]] | ||
Current revision
Crystal structure of the intramolecular trans-sialidase from Ruminococcus gnavus in complex with Siastatin B
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