This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4yt3
From Proteopedia
(Difference between revisions)
m (Protected "4yt3" [edit=sysop:move=sysop]) |
|||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==CYP106A2== | |
| + | <StructureSection load='4yt3' size='340' side='right'caption='[[4yt3]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4yt3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YT3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yt3 OCA], [https://pdbe.org/4yt3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yt3 RCSB], [https://www.ebi.ac.uk/pdbsum/4yt3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yt3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CPXM_PRIMG CPXM_PRIMG] Has the capacity to hydroxylate certain steroids in the 15-beta position. Also hydroxylates progesterone in the 11-alpha and 9-beta position.<ref>PMID:18481342</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CYP106A2 from Bacillus megaterium ATCC 13368 is known as a bacterial steroid hydroxylase which is also capable to hydroxylate a variety of terpenoids. To further analyze the substrate specificity of this enzyme, different resin acids of the abietane- and pimarane-type were tested towards binding and conversion. Product formation could be shown for all tested substrates. Spectroscopic studies revealed type-I binding spectra for isopimaric acid but dehydroabietic acid did not induce a high-spin shift of the enzyme. Interestingly, binding of abietic acid resulted in a type-II difference spectrum typical for nitrogenous inhibitors. Co-crystallization of CYP106A2 with abietic acid and structure determination revealed a bending of the heme-cofactor when abietic acid was bound in the active site. Quantum chemical calculations strongly suggest that this heme distortion is the cause of the unusual spectroscopic characteristics. | ||
| - | + | Crystal structure of CYP106A2 in substrate-free and substrate-bound form.,Janocha S, Carius Y, Hutter M, Lancaster CR, Bernhardt R Chembiochem. 2016 Feb 11. doi: 10.1002/cbic.201500524. PMID:26864272<ref>PMID:26864272</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4yt3" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: Janocha | + | *[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Priestia megaterium]] | ||
| + | [[Category: Bernhardt r]] | ||
| + | [[Category: Carius y]] | ||
| + | [[Category: Janocha S]] | ||
| + | [[Category: Lancaster crd]] | ||
Current revision
CYP106A2
| |||||||||||
