4z2s
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4z2s is ON HOLD Authors: Kantsadi, A.L., Peppa, V.I., Leonidas, D.D. Description: The crystal structure of Sclerotium Rolfsii lectin variant 2 (SSR...) |
|||
| (5 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | The | + | ==The crystal structure of Sclerotium Rolfsii lectin variant 2 (SSR2) in complex with N-acetyl-glucosamine== |
| + | <StructureSection load='4z2s' size='340' side='right'caption='[[4z2s]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4z2s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Athelia_rolfsii Athelia rolfsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z2S FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z2s OCA], [https://pdbe.org/4z2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z2s RCSB], [https://www.ebi.ac.uk/pdbsum/4z2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z2s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0M3KL31_9AGAM A0A0M3KL31_9AGAM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | SRL is a cell wall associated developmental-stage specific lectin secreted by Sclerotium rolfsii, a soil-born pathogenic fungus. SRL displays specificity for TF antigen (Galbeta1-->3GalNAc-alpha-Ser//Thr) expressed in all cancer types and has tumour suppressing effects in vivo. Considering the immense potential of SRL in cancer research, we have generated two variant gene constructs of SRL and expressed in E. coli to refine the sugar specificity and solubility by altering the surface charge. SSR1 and SSR2 are two different recombinant variants of SRL, both of which recognize TF antigen but only SSR1 binds to Tn antigen (GalNAcalpha-Ser/Thr). The glycan array analysis of the variants demonstrated that SSR1 recognizes TF antigen and their derivative with high affinity similar to SRL but showed highest affinity towards the sialylated Tn antigen, unlike SRL. The carbohydrate binding property of SSR2 remains unaltered compared to SRL. The crystal structures of the two variants were determined in free form and in complex with N-acetylglucosamine at 1.7 A and 1.6 A resolution, respectively. Structural analysis highlighted the structural basis of the fine carbohydrate specificity of the two SRL variants and results are in agreement with glycan array analysis. | ||
| - | + | Molecular Cloning, Carbohydrate Specificity and the Crystal Structure of Two Sclerotium rolfsii Lectin Variants.,Peppa VI, Venkat H, Kantsadi AL, Inamdar SR, Bhat GG, Eligar S, Shivanand A, Chachadi VB, Satisha GJ, Swamy BM, Skamnaki VT, Zographos SE, Leonidas DD Molecules. 2015 Jun 12;20(6):10848-65. doi: 10.3390/molecules200610848. PMID:26076107<ref>PMID:26076107</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4z2s" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kantsadi | + | == References == |
| - | [[Category: Leonidas | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Athelia rolfsii]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kantsadi AL]] | ||
| + | [[Category: Leonidas DD]] | ||
| + | [[Category: Peppa VI]] | ||
Current revision
The crystal structure of Sclerotium Rolfsii lectin variant 2 (SSR2) in complex with N-acetyl-glucosamine
| |||||||||||
