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4zb2
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4zb2 is ON HOLD until Paper Publication Authors: Sandy, J. Description: A native form of glucose isomerase collected at room temperature. [[Categor...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==A native form of glucose isomerase collected at room temperature.== | |
| + | <StructureSection load='4zb2' size='340' side='right'caption='[[4zb2]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4zb2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZB2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zb2 OCA], [https://pdbe.org/4zb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zb2 RCSB], [https://www.ebi.ac.uk/pdbsum/4zb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zb2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Dehydration may change the crystal lattice and affect the mosaicity, resolution and quality of X-ray diffraction data. A dehydrating environment can be generated around a crystal in several ways with various degrees of precision and complexity. This study uses a high-precision crystal humidifier/dehumidifier to provide an airstream of known relative humidity in which the crystals are mounted: a precise yet hassle-free approach to altering crystal hydration. A protocol is introduced to assess the impact of crystal dehydration systematically applied to nine experimental crystal systems. In one case, that of glucose isomerase, dehydration triggering a change of space group from I222 to P21212 was observed. This observation is supported by an extended study of the behaviour of the glucose isomerase crystal structure during crystal dehydration. | ||
| - | + | A generic protocol for protein crystal dehydration using the HC1b humidity controller.,Lobley CM, Sandy J, Sanchez-Weatherby J, Mazzorana M, Krojer T, Nowak RP, Sorensen TL Acta Crystallogr D Struct Biol. 2016 May 1;72(Pt 5):629-40. doi:, 10.1107/S2059798316003065. Epub 2016 Apr 26. PMID:27139626<ref>PMID:27139626</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Sandy | + | <div class="pdbe-citations 4zb2" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces rubiginosus]] | ||
| + | [[Category: Sandy J]] | ||
Current revision
A native form of glucose isomerase collected at room temperature.
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