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| | ==Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with CDP-choline== | | ==Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with CDP-choline== |
| - | <StructureSection load='4zcs' size='340' side='right' caption='[[4zcs]], [[Resolution|resolution]] 2.45Å' scene=''> | + | <StructureSection load='4zcs' size='340' side='right'caption='[[4zcs]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zcs]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZCS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zcs]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZCS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CDC:[2-CYTIDYLATE-O-PHOSPHONYLOXYL]-ETHYL-TRIMETHYL-AMMONIUM'>CDC</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zcp|4zcp]], [[4zcq|4zcq]], [[4zcr|4zcr]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CDC:[2-CYTIDYLATE-O-PHOSPHONYLOXYL]-ETHYL-TRIMETHYL-AMMONIUM'>CDC</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF3D7_1316600 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zcs OCA], [https://pdbe.org/4zcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zcs RCSB], [https://www.ebi.ac.uk/pdbsum/4zcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zcs ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Choline-phosphate_cytidylyltransferase Choline-phosphate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.15 2.7.7.15] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zcs OCA], [http://pdbe.org/4zcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zcs RCSB], [http://www.ebi.ac.uk/pdbsum/4zcs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zcs ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8IEE9_PLAF7 Q8IEE9_PLAF7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Choline-phosphate cytidylyltransferase]] | + | [[Category: Large Structures]] |
| - | [[Category: Plaf7]] | + | [[Category: Plasmodium falciparum 3D7]] |
| - | [[Category: Cerdan, R]] | + | [[Category: Cerdan R]] |
| - | [[Category: Guca, E]] | + | [[Category: Guca E]] |
| - | [[Category: Guichou, J F]] | + | [[Category: Guichou J-F]] |
| - | [[Category: Hoh, F]] | + | [[Category: Hoh F]] |
| - | [[Category: Cytidylyltransferase]]
| + | |
| - | [[Category: Enzyme]]
| + | |
| - | [[Category: Malaria]]
| + | |
| - | [[Category: Phosphatidylcholine]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
Q8IEE9_PLAF7
Publication Abstract from PubMed
The development of the malaria parasite, Plasmodium falciparum, in the human erythrocyte, relies on phospholipid metabolism to fulfil the massive need for membrane biogenesis. Phosphatidylcholine (PC) is the most abundant phospholipid in Plasmodium membranes. PC biosynthesis is mainly ensured by the de novo Kennedy pathway that is considered as an antimalarial drug target. The CTP:phosphocholine cytidylyltransferase (CCT) catalyses the rate-limiting step of the Kennedy pathway. Here we report a series of structural snapshots of the PfCCT catalytic domain in its free, substrate- and product-complexed states that demonstrate the conformational changes during the catalytic mechanism. Structural data show the ligand-dependent conformational variations of a flexible lysine. Combined kinetic and ligand-binding analyses confirm the catalytic roles of this lysine and of two threonine residues of the helix alphaE. Finally, we assessed the variations in active site residues between Plasmodium and mammalian CCT which could be exploited for future antimalarial drug design.
Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis.,Guca E, Nagy GN, Hajdu F, Marton L, Izrael R, Hoh F, Yang Y, Vial H, Vertessy BG, Guichou JF, Cerdan R Sci Rep. 2018 Jul 25;8(1):11215. doi: 10.1038/s41598-018-29500-9. PMID:30046154[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Guca E, Nagy GN, Hajdu F, Marton L, Izrael R, Hoh F, Yang Y, Vial H, Vertessy BG, Guichou JF, Cerdan R. Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis. Sci Rep. 2018 Jul 25;8(1):11215. doi: 10.1038/s41598-018-29500-9. PMID:30046154 doi:http://dx.doi.org/10.1038/s41598-018-29500-9
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