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5a2l
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5a2l is ON HOLD Authors: Martinez-Saez, N., Castro-Lopez, J., Valero-Gonzalez, J., Madariaga, D., Companon, I., Somovilla, V.J., Salvado, M., Asensi...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of scFv-SM3 in complex with APD-CGalNAc-RP== | |
| + | <StructureSection load='5a2l' size='340' side='right'caption='[[5a2l]], [[Resolution|resolution]] 1.79Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5a2l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A2L FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a2l OCA], [https://pdbe.org/5a2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a2l RCSB], [https://www.ebi.ac.uk/pdbsum/5a2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a2l ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/HVM32_MOUSE HVM32_MOUSE] [https://www.uniprot.org/uniprot/LV1E_MOUSE LV1E_MOUSE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structural features of MUC1-like glycopeptides bearing the Tn antigen (alpha-O-GalNAc-Ser/Thr) in complex with an anti MUC-1 antibody are reported at atomic resolution. For the alpha-O-GalNAc-Ser derivative, the glycosidic linkage adopts a high-energy conformation, barely populated in the free state. This unusual structure (also observed in an alpha-S-GalNAc-Cys mimic) is stabilized by hydrogen bonds between the peptidic fragment and the sugar. The selection of a particular peptide structure by the antibody is thus propagated to the carbohydrate through carbohydrate/peptide contacts, which force a change in the orientation of the sugar moiety. This seems to be unfeasible in the alpha-O-GalNAc-Thr glycopeptide owing to the more limited flexibility of the side chain imposed by the methyl group. Our data demonstrate the non-equivalence of Ser and Thr O-glycosylation points in molecular recognition processes. These features provide insight into the occurrence in nature of the APDTRP epitope for anti-MUC1 antibodies. | ||
| - | + | Deciphering the Non-Equivalence of Serine and Threonine O-Glycosylation Points: Implications for Molecular Recognition of the Tn Antigen by an anti-MUC1 Antibody.,Martinez-Saez N, Castro-Lopez J, Valero-Gonzalez J, Madariaga D, Companon I, Somovilla VJ, Salvado M, Asensio JL, Jimenez-Barbero J, Avenoza A, Busto JH, Bernardes GJ, Peregrina JM, Hurtado-Guerrero R, Corzana F Angew Chem Int Ed Engl. 2015 Jun 26. doi: 10.1002/anie.201502813. PMID:26118689<ref>PMID:26118689</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5a2l" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Antibody 3D structures|Antibody 3D structures]] |
| - | [[Category: | + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] |
| - | [[Category: Castro-Lopez | + | *[[3D structures of non-human antibody|3D structures of non-human antibody]] |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Jimenez-Barbero | + | </StructureSection> |
| - | [[Category: | + | [[Category: Homo sapiens]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Mus musculus]] |
| - | [[Category: | + | [[Category: Asensio JL]] |
| - | [[Category: | + | [[Category: Avenoza A]] |
| + | [[Category: Bernardes GJL]] | ||
| + | [[Category: Busto JH]] | ||
| + | [[Category: Castro-Lopez J]] | ||
| + | [[Category: Companon I]] | ||
| + | [[Category: Corzana F]] | ||
| + | [[Category: Hurtado-Guerrero R]] | ||
| + | [[Category: Jimenez-Barbero J]] | ||
| + | [[Category: Madariaga D]] | ||
| + | [[Category: Martinez-Saez N]] | ||
| + | [[Category: Peregrina JM]] | ||
| + | [[Category: Salvado M]] | ||
| + | [[Category: Somovilla VJ]] | ||
| + | [[Category: Valero-Gonzalez J]] | ||
Current revision
Crystal structure of scFv-SM3 in complex with APD-CGalNAc-RP
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