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5cnq

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'''Unreleased structure'''
 
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The entry 5cnq is ON HOLD
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==Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA, Mg2+ and Mn2+ ions==
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<StructureSection load='5cnq' size='340' side='right'caption='[[5cnq]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5cnq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CNQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.602&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cnq OCA], [https://pdbe.org/5cnq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cnq RCSB], [https://www.ebi.ac.uk/pdbsum/5cnq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cnq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/G0RYN2_CHATD G0RYN2_CHATD]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We present the crystal structure of the junction-resolving enzyme GEN1 bound to DNA at 2.5 A resolution. The structure of the GEN1 protein reveals it to have an elaborated FEN-XPG family fold that is modified for its role in four-way junction resolution. The functional unit in the crystal is a monomer of active GEN1 bound to the product of resolution cleavage, with an extensive DNA binding interface for both helical arms. Within the crystal lattice, a GEN1 dimer interface juxtaposes two products, whereby they can be reconnected into a four-way junction, the structure of which agrees with that determined in solution. The reconnection requires some opening of the DNA structure at the center, in agreement with permanganate probing and 2-aminopurine fluorescence. The structure shows that a relaxation of the DNA structure accompanies cleavage, suggesting how second-strand cleavage is accelerated to ensure productive resolution of the junction.
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Authors: Liu, Y.J., Freeman, A.D., Declais, A.C., Wilson, T.J., Gartner, A., Lilley, D.M.J.
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Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA.,Liu Y, Freeman AD, Declais AC, Wilson TJ, Gartner A, Lilley DM Cell Rep. 2015 Dec 22;13(11):2565-75. doi: 10.1016/j.celrep.2015.11.042. Epub, 2015 Dec 10. PMID:26686639<ref>PMID:26686639</ref>
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Description: Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA, Mg2+ and Mn2+ ions
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Lilley, D.M.J]]
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<div class="pdbe-citations 5cnq" style="background-color:#fffaf0;"></div>
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[[Category: Declais, A.C]]
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== References ==
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[[Category: Freeman, A.D]]
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<references/>
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[[Category: Liu, Y.J]]
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__TOC__
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[[Category: Gartner, A]]
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</StructureSection>
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[[Category: Wilson, T.J]]
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[[Category: Chaetomium thermophilum]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Declais AC]]
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[[Category: Freeman ADJ]]
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[[Category: Gartner A]]
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[[Category: Lilley DMJ]]
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[[Category: Liu YJ]]
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[[Category: Wilson TJ]]

Current revision

Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA, Mg2+ and Mn2+ ions

PDB ID 5cnq

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