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5l3r

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Structure of the GTPase heterodimer of chloroplast SRP54 and FtsY from Arabidopsis thaliana

Structural highlights

5l3r is a 4 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SR54C_ARATH Involved in cotranslational and post-translational sorting of thylakoid proteins. Binds GTP specifically. Activates the GTPase activity of CPFTSY when bound together. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

The signal recognition particle (SRP) is a ribonucleoprotein complex with a key role in targeting and insertion of membrane proteins. The two SRP GTPases, SRP54 (Ffh in bacteria) and FtsY (SRalpha in eukaryotes), form the core of the targeting complex (TC) regulating the SRP cycle. The architecture of the TC and its stimulation by RNA has been described for the bacterial SRP system while this information is lacking for other domains of life. Here, we present the crystal structures of the GTPase heterodimers of archaeal (Sulfolobus solfataricus), eukaryotic (Homo sapiens), and chloroplast (Arabidopsis thaliana) SRP systems. The comprehensive structural comparison combined with Brownian dynamics simulations of TC formation allows for the description of the general blueprint and of specific adaptations of the quasi-symmetric heterodimer. Our work defines conserved external nucleotide-binding sites for SRP GTPase activation by RNA. Structural analyses of the GDP-bound, post-hydrolysis states reveal a conserved, magnesium-sensitive switch within the I-box. Overall, we provide a general model for SRP cycle regulation by RNA.

Structural Basis for Conserved Regulation and Adaptation of the Signal Recognition Particle Targeting Complex.,Wild K, Bange G, Motiejunas D, Kribelbauer J, Hendricks A, Segnitz B, Wade RC, Sinning I J Mol Biol. 2016 May 27. pii: S0022-2836(16)30184-X. doi:, 10.1016/j.jmb.2016.05.015. PMID:27241309^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tu CJ, Schuenemann D, Hoffman NE. Chloroplast FtsY, chloroplast signal recognition particle, and GTP are required to reconstitute the soluble phase of light-harvesting chlorophyll protein transport into thylakoid membranes. J Biol Chem. 1999 Sep 17;274(38):27219-24. PMID:10480939
↑ Groves MR, Mant A, Kuhn A, Koch J, Dubel S, Robinson C, Sinning I. Functional characterization of recombinant chloroplast signal recognition particle. J Biol Chem. 2001 Jul 27;276(30):27778-86. Epub 2001 May 16. PMID:11356852 doi:http://dx.doi.org/10.1074/jbc.M103470200
↑ Jaru-Ampornpan P, Chandrasekar S, Shan SO. Efficient interaction between two GTPases allows the chloroplast SRP pathway to bypass the requirement for an SRP RNA. Mol Biol Cell. 2007 Jul;18(7):2636-45. Epub 2007 May 2. PMID:17475780 doi:http://dx.doi.org/10.1091/mbc.E07-01-0037
↑ Rutschow H, Ytterberg AJ, Friso G, Nilsson R, van Wijk KJ. Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis. Plant Physiol. 2008 Sep;148(1):156-75. doi: 10.1104/pp.108.124545. Epub 2008 Jul , 16. PMID:18633119 doi:http://dx.doi.org/10.1104/pp.108.124545
↑ Wild K, Bange G, Motiejunas D, Kribelbauer J, Hendricks A, Segnitz B, Wade RC, Sinning I. Structural Basis for Conserved Regulation and Adaptation of the Signal Recognition Particle Targeting Complex. J Mol Biol. 2016 May 27. pii: S0022-2836(16)30184-X. doi:, 10.1016/j.jmb.2016.05.015. PMID:27241309 doi:http://dx.doi.org/10.1016/j.jmb.2016.05.015

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5l3r"

@@ Line 1: / Line 1: @@
 ==Structure of the GTPase heterodimer of chloroplast SRP54 and FtsY from Arabidopsis thaliana==
-<StructureSection load='5l3r' size='340' side='right' caption='[[5l3r]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='5l3r' size='340' side='right'caption='[[5l3r]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5l3r]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L3R FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5l3r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L3R FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l3r OCA], [http://pdbe.org/5l3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l3r RCSB], [http://www.ebi.ac.uk/pdbsum/5l3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l3r ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l3r OCA], [https://pdbe.org/5l3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l3r RCSB], [https://www.ebi.ac.uk/pdbsum/5l3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l3r ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SR54C_ARATH SR54C_ARATH]] Involved in cotranslational and post-translational sorting of thylakoid proteins. Binds GTP specifically. Activates the GTPase activity of CPFTSY when bound together. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids.<ref>PMID:10480939</ref> <ref>PMID:11356852</ref> <ref>PMID:17475780</ref> <ref>PMID:18633119</ref>  [[http://www.uniprot.org/uniprot/CFTSY_ARATH CFTSY_ARATH]] Signal recognition particle receptor protein. Binds GTP specifically. The GTPase activity is inhibited by the N-terminus of the protein until binding to the thylakoid membrane. Activates the GTPase activity of FFC/cpSRP54 when bound to the cpSRP complex. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids. Might be also functionally linked to the Sec translocation machinery.<ref>PMID:10214972</ref> <ref>PMID:10480939</ref> <ref>PMID:12105232</ref> <ref>PMID:17475780</ref> <ref>PMID:18764927</ref> <ref>PMID:19293157</ref>
+[https://www.uniprot.org/uniprot/SR54C_ARATH SR54C_ARATH] Involved in cotranslational and post-translational sorting of thylakoid proteins. Binds GTP specifically. Activates the GTPase activity of CPFTSY when bound together. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids.<ref>PMID:10480939</ref> <ref>PMID:11356852</ref> <ref>PMID:17475780</ref> <ref>PMID:18633119</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5l3r" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Signal recognition particle 3D structures|Signal recognition particle 3D structures]]
+*[[Signal recognition particle receptor 3D structures|Signal recognition particle receptor 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bange, G]]
+[[Category: Arabidopsis thaliana]]
-[[Category: Kribelbauer, J]]
+[[Category: Large Structures]]
-[[Category: Sinning, I]]
+[[Category: Bange G]]
-[[Category: Wild, K]]
+[[Category: Kribelbauer J]]
-[[Category: Co-translational protein targeting]]
+[[Category: Sinning I]]
-[[Category: Gtpase]]
+[[Category: Wild K]]
-[[Category: Protein transport]]
-[[Category: Signal recognition particle]]

5l3r

From Proteopedia

Current revision

Structure of the GTPase heterodimer of chloroplast SRP54 and FtsY from Arabidopsis thaliana

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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