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1pj9

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[[Image:1pj9.jpg|left|200px]]
[[Image:1pj9.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1pj9 |SIZE=350|CAPTION= <scene name='initialview01'>1pj9</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1pj9", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1pj9| PDB=1pj9 | SCENE= }}
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|RELATEDENTRY=[[1cdg|1CDG]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj9 OCA], [http://www.ebi.ac.uk/pdbsum/1pj9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pj9 RCSB]</span>
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}}
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'''Bacillus circulans strain 251 loop mutant 183-195'''
'''Bacillus circulans strain 251 loop mutant 183-195'''
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[[Category: Dijkstra, B W.]]
[[Category: Dijkstra, B W.]]
[[Category: Rozeboom, H J.]]
[[Category: Rozeboom, H J.]]
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[[Category: cyclodextrin]]
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[[Category: Cyclodextrin]]
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[[Category: glycosyltransferase]]
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[[Category: Glycosyltransferase]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:08:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:00:23 2008''
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Revision as of 02:08, 3 May 2008

Image:1pj9.jpg

Template:STRUCTURE 1pj9

Bacillus circulans strain 251 loop mutant 183-195


Overview

Cyclodextrin glycosyltransferase (CGTase) catalyzes the formation of cyclodextrins from starch. Among the CGTases with known three-dimensional structure, Thermoanaerobacterium thermosulfurigenes CGTase has the highest thermostability. By replacing amino acid residues in the B-domain of Bacillus circulans CGTase with those from T. thermosulfurigenes CGTase, we identified a B. circulans CGTase mutant (with N188D and K192R mutations), with a strongly increased activity half-life at 60 degrees C. Asp188 and Arg192 form a salt bridge in T. thermosulfurigenes CGTase. Structural analysis of the B. circulans CGTase mutant revealed that this salt bridge is also formed in the mutant. Thus, the activity half-life of this enzyme can be enhanced by rational protein engineering.

About this Structure

1PJ9 is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.

Reference

Improved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge., Leemhuis H, Rozeboom HJ, Dijkstra BW, Dijkhuizen L, Proteins. 2004 Jan 1;54(1):128-34. PMID:14705029 Page seeded by OCA on Sat May 3 05:08:47 2008

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