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1pjy
From Proteopedia
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[[Image:1pjy.gif|left|200px]] | [[Image:1pjy.gif|left|200px]] | ||
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'''Solution structure of the HIV-1 frameshift inducing stem-loop RNA''' | '''Solution structure of the HIV-1 frameshift inducing stem-loop RNA''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJY OCA]. | |
==Reference== | ==Reference== | ||
Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12888491 12888491] | Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12888491 12888491] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Butcher, S E.]] | [[Category: Butcher, S E.]] | ||
[[Category: Staple, D W.]] | [[Category: Staple, D W.]] | ||
| - | [[Category: | + | [[Category: Frameshift]] |
| - | [[Category: | + | [[Category: Hiv]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Rna structure]] |
| - | [[Category: | + | [[Category: Tetraloop]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:10:17 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:10, 3 May 2008
Solution structure of the HIV-1 frameshift inducing stem-loop RNA
Overview
The translation of reverse transcriptase and other essential viral proteins from the HIV-1 Pol mRNA requires a programmed -1 ribosomal frameshift. This frameshift is induced by two highly conserved elements within the HIV-1 mRNA: a slippery sequence comprised of a UUUUUUA heptamer, and a downstream stem-loop structure. We have determined the structure of the HIV-1 frameshift inducing RNA stem-loop, using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The 22 nucleotide RNA solution structure [root mean squared deviation (r.m.s.d.) = 1.2 A] was determined from 475 nuclear Overhauser effect (NOE)-derived distance restrains, 20 residual dipolar couplings and direct detection of hydrogen bonds via scalar couplings. We find that the frameshift inducing stem-loop is an A-form helix capped by a structured ACAA tetraloop. The ACAA tetraloop is stabilized by an equilateral 5' and 3' stacking pattern, a sheared A-A pair and a cross-strand hydrogen bond. Unexpectedly, the ACAA tetraloop structure is nearly identical to a known tetraloop fold, previously identified in the RNase III recognition site from Saccharomyces cerevisiae.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:12888491 Page seeded by OCA on Sat May 3 05:10:17 2008
Categories: Butcher, S E. | Staple, D W. | Frameshift | Hiv | Nmr | Rna structure | Tetraloop
