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| | ==Crystal structure of phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis== | | ==Crystal structure of phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis== |
| - | <StructureSection load='5mp7' size='340' side='right' caption='[[5mp7]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='5mp7' size='340' side='right'caption='[[5mp7]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5mp7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MP7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5mp7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MP7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prs, MSMEG_5427, MSMEI_5278 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribose-phosphate_diphosphokinase Ribose-phosphate diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.1 2.7.6.1] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mp7 OCA], [https://pdbe.org/5mp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mp7 RCSB], [https://www.ebi.ac.uk/pdbsum/5mp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mp7 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mp7 OCA], [http://pdbe.org/5mp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mp7 RCSB], [http://www.ebi.ac.uk/pdbsum/5mp7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mp7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0R3C8_MYCS2 A0R3C8_MYCS2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Mycs2]] | + | [[Category: Large Structures]] |
| - | [[Category: Ribose-phosphate diphosphokinase]] | + | [[Category: Mycolicibacterium smegmatis MC2 155]] |
| - | [[Category: Donini, S]] | + | [[Category: Donini S]] |
| - | [[Category: Ferraris, D M]] | + | [[Category: Ferraris DM]] |
| - | [[Category: Garavaglia, S]] | + | [[Category: Garavaglia S]] |
| - | [[Category: Miggiano, R]] | + | [[Category: Miggiano R]] |
| - | [[Category: Mori, s]] | + | [[Category: Mori S]] |
| - | [[Category: Rizzi, M]] | + | [[Category: Rizzi M]] |
| - | [[Category: Shibayama, K]] | + | [[Category: Shibayama K]] |
| - | [[Category: Mycobacterium smegmati]]
| + | |
| - | [[Category: Phosphoribosylpyrophosphate synthetase]]
| + | |
| - | [[Category: Prpp synthetase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
A0R3C8_MYCS2
Publication Abstract from PubMed
Mycobacterium smegmatis represents one model for studying the biology of its pathogenic relative Mycobacterium tuberculosis. The structural characterization of a M. tuberculosis ortholog protein can serve as a valid tool for the development of molecules active against the M. tuberculosis target. In this context, we report the biochemical and structural characterization of M. smegmatis phosphoribosylpyrophosphate synthetase (PrsA), the ortholog of M. tuberculosis PrsA, the unique enzyme responsible for the synthesis of phosphoribosylpyrophosphate (PRPP). PRPP is a key metabolite involved in several biosynthetic pathways including those for histidine, tryptophan, nucleotides and decaprenylphosphoryl-arabinose, an essential precursor for the mycobacterial cell wall biosynthesis. Since M. tuberculosis PrsA has been validated as a drug target for the development of antitubercular agents, the data presented here will add to the knowledge of the mycobacterial enzyme and could contribute to the development of M. tuberculosis PrsA inhibitors of potential pharmacological interest.
Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis.,Donini S, Garavaglia S, Ferraris DM, Miggiano R, Mori S, Shibayama K, Rizzi M PLoS One. 2017 Apr 18;12(4):e0175815. doi: 10.1371/journal.pone.0175815., eCollection 2017. PMID:28419153[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Donini S, Garavaglia S, Ferraris DM, Miggiano R, Mori S, Shibayama K, Rizzi M. Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis. PLoS One. 2017 Apr 18;12(4):e0175815. doi: 10.1371/journal.pone.0175815., eCollection 2017. PMID:28419153 doi:http://dx.doi.org/10.1371/journal.pone.0175815
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