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6i2z

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Isolated globin domain of the Bordetella pertussis globin-coupled sensor

Structural highlights

6i2z is a 2 chain structure with sequence from Bordetella pertussis Tohama I. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q7VTL8_BORPE

Publication Abstract from PubMed

AIMS: Structural and functional characterization of the globin coupled sensors (GCS) of Azotobacter vinelandii (AvGReg) and Bordetella pertussis (BpeGReg). RESULTS: UV/Vis and RR spectroscopies confirm the presence in AvGReg and BpeGReg of a globin domain capable of reversible gaseous ligand binding. In AvGReg an influence of the transmitter domain on the heme proximal region of the globin domain can be seen, and k'CO is higher than for other GCSs. The O2 binding kinetics suggest the presence of an open and a closed conformation. As for BpeGReg, the fully oxygenated AvGReg show a very high DGC activity. The CO rebinding to BpeGReg indicates that intra- and intermolecular interactions influence the ligand binding. The globin domains of both proteins (AvGReg-Gb and BpeGreg-Gb*) share the same GCS fold, a similar proximal but a different distal side structure. They homodimerize through a G-H helical bundle as in other GCS. However, BpeGReg-Gb* shows also a second dimerization mode. INNOVATION: This paper extends our knowledge on the GCS proteins and contributes to a better understanding of the GCSs' role in the formation of bacterial biofilms. CONCLUSIONS: AvGReg and BpeGreg conform to the GCS family, share a similar overall structure but they have different properties in terms of the ligand binding. In particular, AvGreg shows an open and closed conformation that in the latter form will very tightly bind oxygen. BpeGReg has only one closed conformation. In both proteins, it is the fully oxygenated GCS form that catalyzes the production of the second messenger.

Structural and functional characterization of the globin-coupled sensors of Azotobacter vinelandii and Bordetella pertussis.,Germani F, Nardini M, De Schutter A, Cuypers B, Berghmans H, Van Hauwaert ML, Stefano B, Mozzarelli A, Moens L, Van Doorslaer S, Bolognesi M, Pesce A, Dewilde S Antioxid Redox Signal. 2019 Sep 27. doi: 10.1089/ars.2018.7690. PMID:31559835^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Germani F, Nardini M, De Schutter A, Cuypers B, Berghmans H, Van Hauwaert ML, Stefano B, Mozzarelli A, Moens L, Van Doorslaer S, Bolognesi M, Pesce A, Dewilde S. Structural and functional characterization of the globin-coupled sensors of Azotobacter vinelandii and Bordetella pertussis. Antioxid Redox Signal. 2019 Sep 27. doi: 10.1089/ars.2018.7690. PMID:31559835 doi:http://dx.doi.org/10.1089/ars.2018.7690

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6i2z"

@@ Line 3: / Line 3: @@
 <StructureSection load='6i2z' size='340' side='right'caption='[[6i2z]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6i2z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I2Z FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6i2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis_Tohama_I Bordetella pertussis Tohama I]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I2Z FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uiq|4uiq]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2z OCA], [http://pdbe.org/6i2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i2z RCSB], [http://www.ebi.ac.uk/pdbsum/6i2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2z ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2z OCA], [https://pdbe.org/6i2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i2z RCSB], [https://www.ebi.ac.uk/pdbsum/6i2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2z ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q7VTL8_BORPE Q7VTL8_BORPE]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Bordetella pertussis Tohama I]]
 [[Category: Large Structures]]
-[[Category: Berghmans, H]]
+[[Category: Berghmans H]]
-[[Category: Bolognesi, M]]
+[[Category: Bolognesi M]]
-[[Category: Bruno, S]]
+[[Category: Bruno S]]
-[[Category: Cuypers, B]]
+[[Category: Cuypers B]]
-[[Category: Dewilde, S]]
+[[Category: De Schutter A]]
-[[Category: Doorslaer, S Van]]
+[[Category: Dewilde S]]
-[[Category: Germani, F]]
+[[Category: Germani F]]
-[[Category: Hauwaert, M L.Van]]
+[[Category: Moens L]]
-[[Category: Moens, L]]
+[[Category: Mozzarelli A]]
-[[Category: Mozzarelli, A]]
+[[Category: Pesce A]]
-[[Category: Pesce, A]]
+[[Category: Van Doorslaer S]]
-[[Category: Schutter, A De]]
+[[Category: Van Hauwaert M-L]]
-[[Category: Biofilm formation]]
-[[Category: C-di-gmp and enzyme specificity]]
-[[Category: Heme-based sensor]]
-[[Category: Oxygen affinity]]
-[[Category: Transferase]]

6i2z

From Proteopedia

Current revision

Isolated globin domain of the Bordetella pertussis globin-coupled sensor

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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